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The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling.
Liu, Yulu C; Couzens, Amber L; Deshwar, Ashish R; B McBroom-Cerajewski, Linda D; Zhang, Xiaoyun; Puviindran, Vijitha; Scott, Ian C; Gingras, Anne-Claude; Hui, Chi-Chung; Angers, Stephane.
Afiliación
  • Liu YC; Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario M5S 3M2, Canada.
  • Couzens AL; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
  • Deshwar AR; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
  • B McBroom-Cerajewski LD; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada.
  • Zhang X; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
  • Puviindran V; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
  • Scott IC; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada. Program in Developmental and Stem Cell Biology, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.
  • Gingras AC; Lunenfeld-Tanenbaum Research Institute, Mount Sinai Hospital, Toronto, Ontario M5G 1X5, Canada. Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada.
  • Hui CC; Department of Molecular Genetics, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada. Program in Developmental and Stem Cell Biology, Peter Gilgan Centre for Research and Learning, The Hospital for Sick Children, Toronto, Ontario M5G 0A4, Canada.
  • Angers S; Department of Pharmaceutical Sciences, Leslie Dan Faculty of Pharmacy, University of Toronto, Toronto, Ontario M5S 3M2, Canada. Department of Biochemistry, Faculty of Medicine, University of Toronto, Toronto, Ontario M5S 1A8, Canada. stephane.angers@utoronto.ca.
Sci Signal ; 7(355): ra117, 2014 Dec 09.
Article en En | MEDLINE | ID: mdl-25492966
The primary cilium is required for Hedgehog (Hh) signaling in vertebrates. Hh leads to ciliary accumulation and activation of the transmembrane protein Smoothened (Smo) and affects the localization of several pathway components, including the Gli family of transcriptional regulators, within different regions of primary cilia. Genetic analysis indicates that the kinesin protein Kif7 both promotes and inhibits mouse Hh signaling. Using mass spectrometry, we identified liprin-α1 (PPFIA1) and the protein phosphatase PP2A as Kif7-interacting proteins, and we showed that they were important for the trafficking of Kif7 and Gli proteins to the tips of cilia and for the transcriptional output of Hh signaling. Our results suggested that PPFIA1 functioned with PP2A to promote the dephosphorylation of Kif7, triggering Kif7 localization to the tips of primary cilia and promoting Gli transcriptional activity.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pez Cebra / Transducción de Señal / Proteínas / Cinesinas / Proteínas de Pez Cebra / Proteínas Adaptadoras Transductoras de Señales / Proteínas Hedgehog / Proteína Fosfatasa 2 Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Sci Signal Asunto de la revista: CIENCIA / FISIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Pez Cebra / Transducción de Señal / Proteínas / Cinesinas / Proteínas de Pez Cebra / Proteínas Adaptadoras Transductoras de Señales / Proteínas Hedgehog / Proteína Fosfatasa 2 Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: Sci Signal Asunto de la revista: CIENCIA / FISIOLOGIA Año: 2014 Tipo del documento: Article País de afiliación: Canadá