The PPFIA1-PP2A protein complex promotes trafficking of Kif7 to the ciliary tip and Hedgehog signaling.
Sci Signal
; 7(355): ra117, 2014 Dec 09.
Article
en En
| MEDLINE
| ID: mdl-25492966
The primary cilium is required for Hedgehog (Hh) signaling in vertebrates. Hh leads to ciliary accumulation and activation of the transmembrane protein Smoothened (Smo) and affects the localization of several pathway components, including the Gli family of transcriptional regulators, within different regions of primary cilia. Genetic analysis indicates that the kinesin protein Kif7 both promotes and inhibits mouse Hh signaling. Using mass spectrometry, we identified liprin-α1 (PPFIA1) and the protein phosphatase PP2A as Kif7-interacting proteins, and we showed that they were important for the trafficking of Kif7 and Gli proteins to the tips of cilia and for the transcriptional output of Hh signaling. Our results suggested that PPFIA1 functioned with PP2A to promote the dephosphorylation of Kif7, triggering Kif7 localization to the tips of primary cilia and promoting Gli transcriptional activity.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Pez Cebra
/
Transducción de Señal
/
Proteínas
/
Cinesinas
/
Proteínas de Pez Cebra
/
Proteínas Adaptadoras Transductoras de Señales
/
Proteínas Hedgehog
/
Proteína Fosfatasa 2
Tipo de estudio:
Prognostic_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Sci Signal
Asunto de la revista:
CIENCIA
/
FISIOLOGIA
Año:
2014
Tipo del documento:
Article
País de afiliación:
Canadá