Isolation and characterization of a novel arenavirus harbored by Rodents and Shrews in Zhejiang province, China.
Virology
; 476: 37-42, 2015 Feb.
Article
en En
| MEDLINE
| ID: mdl-25506671
To determine the biodiversity of arenaviruses in China, we captured and screened rodents and shrews in Wenzhou city, Zhejiang province, a locality where hemorrhagic fever diseases are endemic in humans. Accordingly, arenaviruses were detected in 42 of 351 rodents from eight species, and in 12 of 272 Asian house shrews (Suncus murinus), by RT-PCR targeting the L segment. From these, a single arenavirus was successfully isolated in cell culture. The virion particles exhibited a typical arenavirus morphology under transmission electron microscopy. Comparison of the S and L segment sequences revealed high levels of nucleotide (>32.2% and >39.6%) and amino acid (>28.8% and >43.8%) sequence differences from known arenaviruses, suggesting that it represents a novel arenavirus, which we designated Wenzhou virus (WENV). Phylogenetic analysis revealed that all WENV strains harbored by both rodents and Asian house shrews formed a distinct lineage most closely related to Old World arenaviruses.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Musarañas
/
Arenavirus
/
Muridae
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Virology
Año:
2015
Tipo del documento:
Article