Planarian myosin essential light chain is involved in the formation of brain lateral branches during regeneration.
Mol Genet Genomics
; 290(4): 1277-85, 2015 Aug.
Article
en En
| MEDLINE
| ID: mdl-25585662
ABSTRACT
The myosin essential light chain (ELC) is a structure component of the actomyosin cross-bridge, however, the functions in the central nervous system (CNS) development and regeneration remain poorly understood. Planarian Dugesia japonica has revealed fundamental mechanisms and unique aspects of neuroscience and neuroregeneration. In this study, the cDNA DjElc, encoding a planarian essential light chain of myosin, was identified from the planarian Dugesia japonica cDNA library. It encodes a deduced protein with highly conserved functionally domains EF-Hand and Ca(2+) binding sites that shares significant similarity with other members of ELC. Whole mount in situ hybridization studies show that DjElc expressed in CNS during embryonic development and regeneration of adult planarians. Loss of function of DjElc by RNA interference during planarian regeneration inhibits brain lateral branches regeneration completely. In conclusion, these results demonstrated that DjElc is required for maintenance of neurons and neurite outgrowth, particularly for involving the brain later branch regeneration.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Planarias
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Regeneración
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Encéfalo
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Proteínas del Helminto
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Cadenas Ligeras de Miosina
Límite:
Animals
Idioma:
En
Revista:
Mol Genet Genomics
Asunto de la revista:
BIOLOGIA MOLECULAR
/
GENETICA
Año:
2015
Tipo del documento:
Article