Your browser doesn't support javascript.
loading
Isoflurane does not protect from brain death-associated aggravation of cold hepatic ischemia-reperfusion injury.
Strowitzki, Moritz J; Moussavian, Mohammed R; Keppler, Ulrich; Schilling, Martin K; Menger, Michael D; von Heesen, Maximilian.
Afiliación
  • Strowitzki MJ; Department of General, Visceral and Transplant Surgery, University of Heidelberg, Heidelberg, Germany.
  • Moussavian MR; Department of General, Vascular and Pediatric Surgery, Saarland University Hospital, Homburg, Germany.
  • Keppler U; Department of General, Visceral and Transplant Surgery, University of Heidelberg, Heidelberg, Germany.
  • Schilling MK; Klinik St. Anna, Ärztehaus Lützelmatt, Luzern, Switzerland.
  • Menger MD; Institute for Clinical & Experimental Surgery, Saarland University, Saarbrücken, Germany.
  • von Heesen M; Department of General, Vascular and Pediatric Surgery, Saarland University Hospital, Homburg, Germany.
Ann Transplant ; 20: 36-43, 2015 Jan 19.
Article en En | MEDLINE | ID: mdl-25598073
BACKGROUND: Previous studies have shown that brain death aggravates cold ischemia-reperfusion injury in liver transplantation. Isoflurane, a volatile anesthetic, has been indicated to reduce warm hepatic ischemia-reperfusion injury. Herein, we studied in Sprague-Dawley rats whether isoflurane is capable of ameliorating brain death-associated aggravation of cold hepatic ischemia-reperfusion injury. MATERIAL AND METHODS: Brain-dead animals were treated for 30 min with isoflurane (MAC 1.5%; n=8). Animals without isoflurane treatment served as controls (n=8). Another 13 animals without induction of brain death served as sham controls. After a 4-h period portal venous blood perfusion, hepatic microcirculation and bile flow were determined. Livers were recovered and stored for 24 h in 4°C cold HTK solution, followed by reperfusion with 37°C Krebs-Henseleit-buffer for 60 min. Liver enzymes in the effluent and bile flow were analyzed. Hepatocellular morphology was determined by histology and immunohistochemistry. RESULTS: Brain death reduced portal venous blood perfusion and bile flow, induced heme oxygenase-1 (HO-1), and resulted in hepatocellular damage. Isoflurane treatment did not prevent the reduction of portal venous blood perfusion or bile flow or the induction of HO-1. Accordingly, isoflurane was not capable of reducing the hepatocellular injury. CONCLUSIONS: Isoflurane does not protect from brain death-associated aggravation of cold hepatic ischemia-reperfusion injury.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Daño por Reperfusión / Isoflurano / Hígado Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Ann Transplant Asunto de la revista: TRANSPLANTE Año: 2015 Tipo del documento: Article País de afiliación: Alemania

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Daño por Reperfusión / Isoflurano / Hígado Tipo de estudio: Risk_factors_studies Límite: Animals / Humans Idioma: En Revista: Ann Transplant Asunto de la revista: TRANSPLANTE Año: 2015 Tipo del documento: Article País de afiliación: Alemania