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The JAK2 inhibitor AZD1480 inhibits hepatitis A virus replication in Huh7 cells.
Jiang, Xia; Kanda, Tatsuo; Nakamoto, Shingo; Saito, Kengo; Nakamura, Masato; Wu, Shuang; Haga, Yuki; Sasaki, Reina; Sakamoto, Naoya; Shirasawa, Hiroshi; Okamoto, Hiroaki; Yokosuka, Osamu.
Afiliación
  • Jiang X; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Kanda T; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan. Electronic address: kandat-cib@umin.ac.jp.
  • Nakamoto S; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan; Department of Molecular Virology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Saito K; Department of Molecular Virology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Nakamura M; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Wu S; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Haga Y; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Sasaki R; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Sakamoto N; Department of Gastroenterology and Hepatology, Hokkaido University, Sapporo, Japan.
  • Shirasawa H; Department of Molecular Virology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
  • Okamoto H; Division of Virology, Department of Infection and Immunity, Jichi Medical University School of Medicine, Shimotsuke, Japan.
  • Yokosuka O; Department of Gastroenterology and Nephrology, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-ku, Chiba 260-8670, Japan.
Biochem Biophys Res Commun ; 458(4): 908-12, 2015 Mar 20.
Article en En | MEDLINE | ID: mdl-25704089
The JAK2 inhibitor AZD1480 has been reported to inhibit La protein expression. We previously demonstrated that the inhibition of La expression could inhibit hepatitis A virus (HAV) internal ribosomal entry-site (IRES)-mediated translation and HAV replication in vitro. In this study, we analyzed the effects of AZD1480 on HAV IRES-mediated translation and replication. HAV IRES-mediated translation in COS7-HAV-IRES cells was inhibited by 0.1-1 µM AZD1480, a dosage that did not affect cell viability. Results showed a significant reduction in intracellular HAV HA11-1299 genotype IIIA RNA levels in Huh7 cells treated with AZD1480. Furthermore, AZD1480 inhibited the expression of phosphorylated-(Tyr-705)-signal transducer and activator of transcription 3 (STAT3) and La in Huh7 cells. Therefore, we propose that AZD1480 can inhibit HAV IRES activity and HAV replication through the inhibition of the La protein.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Antivirales / Pirazoles / Pirimidinas / Replicación Viral / Virus de la Hepatitis A / Janus Quinasa 2 / Hepatitis A Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2015 Tipo del documento: Article País de afiliación: Japón
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Antivirales / Pirazoles / Pirimidinas / Replicación Viral / Virus de la Hepatitis A / Janus Quinasa 2 / Hepatitis A Límite: Animals / Humans Idioma: En Revista: Biochem Biophys Res Commun Año: 2015 Tipo del documento: Article País de afiliación: Japón