Qualifying label components for effective biosensing using advanced high-throughput SEIRA methodology.
Phys Chem Chem Phys
; 17(14): 9471-9, 2015 Apr 14.
Article
en En
| MEDLINE
| ID: mdl-25765006
ABSTRACT
The need for technological progress in bio-diagnostic assays of high complexity requires both fundamental research and constructing efforts on nano-scaled assay recognition elements that can provide unique selectivity and design-enhanced sensitivity features. Nanoparticle induced sensitivity enhancement and its application related to multiplexed capability Surface-Enhanced InfraRed Absorption (SEIRA) assay formats are well suitable for these purposes. The potential of diverse fluorophore-antibody conjugates, being chemisorbed onto low-cost gold nanoparticulate SEIRA substrates, has been explored with respect to their spectral discriminability. These novel biolabels deliver molecular SEIRA fingerprints that have been successfully analyzed by both uni- and multivariate analyzing tools, to discriminate their multiplexing capabilities. We show that this robust spectral encoding via SEIRA fingerprints opens up new opportunities for a fast, reliable and multiplexed high-end screening in biodiagnostics.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espectrofotometría Infrarroja
/
Inmunoensayo
/
Técnicas Biosensibles
/
Nanotecnología
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Nanopartículas del Metal
/
Anticuerpos Inmovilizados
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Oro
Límite:
Humans
Idioma:
En
Revista:
Phys Chem Chem Phys
Asunto de la revista:
BIOFISICA
/
QUIMICA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Alemania