Red and far-red fluorescent dyes for the characterization of head and neck cancer at the cellular level.
J Oral Pathol Med
; 44(10): 831-41, 2015 Nov.
Article
en En
| MEDLINE
| ID: mdl-25779631
ABSTRACT
BACKGROUND:
Primary upper aerodigestive tract malignancy remains a cancer having a poor prognosis, despite current progress in treatment, due to a generally late diagnosis.OBJECTIVES:
We conducted a preliminary assessment of five dyes approved for human use for the imaging of head and neck tissues at the cellular level, which could be considered for clinical examination.METHODS:
We investigated fluorescence endomicroscopic images on fresh samples obtained from head and neck surgeries after staining with hypericin, methylene blue, toluidine blue, patent blue or indocyanine green to provide a preliminary consideration as to whether these images contain enough information for identification of non-pathologic and pathologic tissues. The distribution pattern of dye has been examined using probe-based confocal laser endomicroscopy (pCLE) in ex vivo specimens and compared with corresponding histology.RESULTS:
In most samples, the image quality provided by pCLE with both dyes allowed pathologists to recognize histological characteristics to identify the tissues.CONCLUSION:
The combination of pCLE imaging with these dyes provides interpretable images close to conventional histology; a promising clinical tool to assist physicians in examination of upper aerodigestive tract, as long as depth imaging issues can be overcome.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Colorantes Fluorescentes
/
Neoplasias de Cabeza y Cuello
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
J Oral Pathol Med
Asunto de la revista:
ODONTOLOGIA
/
PATOLOGIA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Francia