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Identification, characterization and full-length sequence analysis of a novel dsRNA virus isolated from the arboreal ant Camponotus yamaokai.
Koyama, Satoshi; Urayama, Syun-Ichi; Ohmatsu, Tsutomu; Sassa, Yukiko; Sakai, Chihiro; Takata, Mamoru; Hayashi, Shinya; Nagai, Makoto; Furuya, Tetsuya; Moriyama, Hiromitsu; Satoh, Toshiyuki; Ono, Shin-Ichi; Mizutani, Testuya.
Afiliación
  • Koyama S; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Urayama S; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Ohmatsu T; 2Research and Education Center for Prevention of Global Infectious Disease of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Sassa Y; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Sakai C; 3Faculty of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Takata M; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Hayashi S; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Nagai M; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Furuya T; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Moriyama H; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Satoh T; 1Graduate School of Agriculture, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
  • Ono S; 4Department of Fisheries, School of Marine Science and Technology, Tokai University, 3-20-1 Orido, Shimizu, Shizuoka, Japan.
  • Mizutani T; 2Research and Education Center for Prevention of Global Infectious Disease of Animals, Tokyo University of Agriculture and Technology, 3-5-8 Saiwai, Fuchu, Tokyo, Japan.
J Gen Virol ; 96(Pt 7): 1930-7, 2015 Jul.
Article en En | MEDLINE | ID: mdl-25787938
A novel dsRNA virus was identified from the arboreal ant Camponotus yamaokai. The complete nucleotide sequence analysis of the virus revealed that the virus consisted of 5704 bp with two ORFs. ORF1 (3084 nt) encoded a putative capsid protein. ORF2 (1977 nt) encoded a viral RNA-dependent RNA polymerase (RdRp). ORF2 could be translated as a fusion with the ORF1 product by a - 1 frameshift in the overlapping ORF1. Phylogenetic analyses based on the RdRp revealed that the virus from C. yamaokai was most likely a novel totivirus, but it was not closely related to the previously known totiviruses in arthropods. Transmission electron microscopy revealed isometric virus particles of ~30 nm diameter in the cytoplasm, which was consistent with the characteristics of the family Totiviridae. The virus was detected by reverse transcription-PCR in all caste members and developmental stages of ants, including eggs, larvae, pupae, adult workers, alates (male and female) and queens. To our knowledge, this is the first report of a member of the family Totiviridae in a hymenopteran; the virus was designated Camponotus yamaokai virus.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Hormigas / Virus ARN / ARN Bicatenario / ARN Viral / Genoma Viral Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Gen Virol Año: 2015 Tipo del documento: Article País de afiliación: Japón
Texto completo
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Hormigas / Virus ARN / ARN Bicatenario / ARN Viral / Genoma Viral Tipo de estudio: Diagnostic_studies / Prognostic_studies Límite: Animals Idioma: En Revista: J Gen Virol Año: 2015 Tipo del documento: Article País de afiliación: Japón