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Inference of the Arabidopsis lateral root gene regulatory network suggests a bifurcation mechanism that defines primordia flanking and central zones.
Lavenus, Julien; Goh, Tatsuaki; Guyomarc'h, Soazig; Hill, Kristine; Lucas, Mikael; Voß, Ute; Kenobi, Kim; Wilson, Michael H; Farcot, Etienne; Hagen, Gretchen; Guilfoyle, Thomas J; Fukaki, Hidehiro; Laplaze, Laurent; Bennett, Malcolm J.
Afiliación
  • Lavenus J; Institut de Recherche pour le Développement, UMR DIADE, 34394 Montpellier cedex 5, France Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.
  • Goh T; Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
  • Guyomarc'h S; Université de Montpellier, UMR DIADE, 34394 Montpellier cedex 5, France.
  • Hill K; Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.
  • Lucas M; Institut de Recherche pour le Développement, UMR DIADE, 34394 Montpellier cedex 5, France.
  • Voß U; Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.
  • Kenobi K; Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.
  • Wilson MH; Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom.
  • Farcot E; Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom Inria, Virtual Plants Team, 34095 Montpellier cedex 5, France.
  • Hagen G; University of Missouri, Columbia, Missouri 65211.
  • Guilfoyle TJ; University of Missouri, Columbia, Missouri 65211.
  • Fukaki H; Department of Biology, Graduate School of Science, Kobe University, Kobe 657-8501, Japan.
  • Laplaze L; Institut de Recherche pour le Développement, UMR DIADE, 34394 Montpellier cedex 5, France laurent.laplaze@ird.fr bennett@nottingham.ac.uk.
  • Bennett MJ; Centre for Plant Integrative Biology, School of Biosciences, University of Nottingham, Loughborough, Leicestershire LE12 5RD, United Kingdom laurent.laplaze@ird.fr bennett@nottingham.ac.uk.
Plant Cell ; 27(5): 1368-88, 2015 May.
Article en En | MEDLINE | ID: mdl-25944102
A large number of genes involved in lateral root (LR) organogenesis have been identified over the last decade using forward and reverse genetic approaches in Arabidopsis thaliana. Nevertheless, how these genes interact to form a LR regulatory network largely remains to be elucidated. In this study, we developed a time-delay correlation algorithm (TDCor) to infer the gene regulatory network (GRN) controlling LR primordium initiation and patterning in Arabidopsis from a time-series transcriptomic data set. The predicted network topology links the very early-activated genes involved in LR initiation to later expressed cell identity markers through a multistep genetic cascade exhibiting both positive and negative feedback loops. The predictions were tested for the key transcriptional regulator AUXIN RESPONSE FACTOR7 node, and over 70% of its targets were validated experimentally. Intriguingly, the predicted GRN revealed a mutual inhibition between the ARF7 and ARF5 modules that would control an early bifurcation between two cell fates. Analyses of the expression pattern of ARF7 and ARF5 targets suggest that this patterning mechanism controls flanking and central zone specification in Arabidopsis LR primordia.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Factores de Transcripción / Arabidopsis / Raíces de Plantas / Proteínas de Arabidopsis / Proteínas de Unión al ADN / Redes Reguladoras de Genes / Transcriptoma Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Factores de Transcripción / Arabidopsis / Raíces de Plantas / Proteínas de Arabidopsis / Proteínas de Unión al ADN / Redes Reguladoras de Genes / Transcriptoma Tipo de estudio: Prognostic_studies Idioma: En Revista: Plant Cell Asunto de la revista: BOTANICA Año: 2015 Tipo del documento: Article País de afiliación: Reino Unido