Expression and Purification of RNA-Protein Complexes in Escherichia coli.
Methods Mol Biol
; 1316: 25-31, 2015.
Article
en En
| MEDLINE
| ID: mdl-25967050
ABSTRACT
For structural, biochemical or pharmacological studies, it is required to have pure RNA in large quantities. We previously devised a generic approach that allows efficient in vivo expression of recombinant RNA in Escherichia coli. We have extended the "tRNA scaffold" method to RNA/protein co-expression in order to express and purify RNA by affinity in native condition. As a proof-of-concept, we present the expression and the purification of the AtRNA-mala in complex with the MS2 coat protein.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ARN
/
Proteínas
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Expresión Génica
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Escherichia coli
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2015
Tipo del documento:
Article
País de afiliación:
Reino Unido