Clinical applications of next-generation sequencing in histocompatibility and transplantation.
Curr Opin Organ Transplant
; 20(4): 461-7, 2015 Aug.
Article
en En
| MEDLINE
| ID: mdl-26107967
ABSTRACT
PURPOSE OF REVIEW Next-generation sequencing (NGS) can overcome traditional methodological barriers to facilitate detailed studies of large genomes. Here, we summarize recent NGS-based developments in histocompatibility and transplantation, and highlight the dynamic range of clinical applications achievable on this platform. RECENT FINDINGS:
Multiple NGS-based protocols have been established to achieve unambiguous human leukocyte antigen genotyping. These methods are presently engaged to serve the high-throughput demand of large bone marrow registries; however, the scalable nature of NGS makes it an equally attractive technology for select applications within solid organ transplantation. Recently, the exquisite sensitivity of NGS has been leveraged to perform noninvasive allograft monitoring by tracking the dynamics of donor-derived cell-free DNA. Further, NGS-based T-cell receptor and immunoglobulin heavy chain repertoire profiling appear to be useful in clarifying disease-specific diagnoses in certain complex allograft pathology; detecting/quantifying minimal residual disease following allogeneic stem cell transplantation; and tracking donor-reactive T cells to understand the mechanism of tolerance in kidney transplant recipients.SUMMARY:
NGS is superior to classical Sanger sequencing in its throughput, sensitivity, and the ability to provide phase-defined sequence data. These unique properties allow its broad application to diverse areas in clinical transplantation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Secuenciación de Nucleótidos de Alto Rendimiento
/
Histocompatibilidad
Tipo de estudio:
Guideline
Límite:
Humans
Idioma:
En
Revista:
Curr Opin Organ Transplant
Año:
2015
Tipo del documento:
Article
País de afiliación:
Canadá