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Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells.
Paull, Daniel; Sevilla, Ana; Zhou, Hongyan; Hahn, Aana Kim; Kim, Hesed; Napolitano, Christopher; Tsankov, Alexander; Shang, Linshan; Krumholz, Katie; Jagadeesan, Premlatha; Woodard, Chris M; Sun, Bruce; Vilboux, Thierry; Zimmer, Matthew; Forero, Eliana; Moroziewicz, Dorota N; Martinez, Hector; Malicdan, May Christine V; Weiss, Keren A; Vensand, Lauren B; Dusenberry, Carmen R; Polus, Hannah; Sy, Karla Therese L; Kahler, David J; Gahl, William A; Solomon, Susan L; Chang, Stephen; Meissner, Alexander; Eggan, Kevin; Noggle, Scott A.
Afiliación
  • Paull D; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Sevilla A; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Zhou H; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Hahn AK; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Kim H; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Napolitano C; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Tsankov A; The Broad Institute, Cambridge, Massachusetts, USA.
  • Shang L; The Harvard Stem Cell Institute, Harvard University, Cambridge, Massachusetts, USA.
  • Krumholz K; Department of Stem Cell and Regenerative Biology, Harvard University, Cambridge, Massachusetts, USA.
  • Jagadeesan P; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Woodard CM; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Sun B; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Vilboux T; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Zimmer M; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Forero E; Section on Human Biochemical Genetics, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA.
  • Moroziewicz DN; Division of Medical Genomics, Inova Translational Medicine Institute, Inova Health System, Falls Church, Virginia, USA.
  • Martinez H; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Malicdan MC; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Weiss KA; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Vensand LB; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Dusenberry CR; Section on Human Biochemical Genetics, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA.
  • Polus H; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Sy KT; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Kahler DJ; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Gahl WA; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Solomon SL; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Chang S; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
  • Meissner A; Section on Human Biochemical Genetics, Medical Genetics Branch, National Human Genome Research Institute, National Institutes of Health, Bethesda, Maryland, USA.
  • Eggan K; NIH Undiagnosed Diseases Program, Common Fund, Office of the Director, National Institute of Health and National Human Genome Research Institute, National Institute of Health, Bethesda, Maryland, USA.
  • Noggle SA; The New York Stem Cell Foundation Research Institute, New York, New York, USA.
Nat Methods ; 12(9): 885-92, 2015 Sep.
Article en En | MEDLINE | ID: mdl-26237226
ABSTRACT
Induced pluripotent stem cells (iPSCs) are an essential tool for modeling how causal genetic variants impact cellular function in disease, as well as an emerging source of tissue for regenerative medicine. The preparation of somatic cells, their reprogramming and the subsequent verification of iPSC pluripotency are laborious, manual processes limiting the scale and reproducibility of this technology. Here we describe a modular, robotic platform for iPSC reprogramming enabling automated, high-throughput conversion of skin biopsies into iPSCs and differentiated cells with minimal manual intervention. We demonstrate that automated reprogramming and the pooled selection of polyclonal pluripotent cells results in high-quality, stable iPSCs. These lines display less line-to-line variation than either manually produced lines or lines produced through automation followed by single-colony subcloning. The robotic platform we describe will enable the application of iPSCs to population-scale biomedical problems including the study of complex genetic diseases and the development of personalized medicines.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Robótica / Separación Celular / Técnicas Analíticas Microfluídicas / Células Madre Pluripotentes Inducidas / Técnicas de Cultivo Celular por Lotes Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Robótica / Separación Celular / Técnicas Analíticas Microfluídicas / Células Madre Pluripotentes Inducidas / Técnicas de Cultivo Celular por Lotes Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2015 Tipo del documento: Article País de afiliación: Estados Unidos