Imaging Acute Neuromuscular Explants from Thy1 Mouse Lines.
Cold Spring Harb Protoc
; 2015(9): pdb.prot087692, 2015 Sep 01.
Article
en En
| MEDLINE
| ID: mdl-26330628
ABSTRACT
Because core facilities that generate transgenic founder mice for a reasonable fee are now available at most major research institutions, generating new Thy1-XFP transgenic animals (in which XFP stands for any fluorescent protein) is an option even for relatively small laboratories. Here, we provide a protocol for screening offspring of Thy1 transgenic founders. Acute neuromuscular explants are obtained from 3-wk-old F1 mice that have been produced by crossing Thy1 transgenic founders and commercially obtained inbred mice. Thy1-driven expression is detected by fluorescence microscopy.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Expresión Génica
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Pruebas Genéticas
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Crioultramicrotomía
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Antígenos Thy-1
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Músculos
Límite:
Animals
Idioma:
En
Revista:
Cold Spring Harb Protoc
Año:
2015
Tipo del documento:
Article