Optogenetic Control of Gene Expression in Drosophila.
PLoS One
; 10(9): e0138181, 2015.
Article
en En
| MEDLINE
| ID: mdl-26383635
ABSTRACT
To study the molecular mechanism of complex biological systems, it is important to be able to artificially manipulate gene expression in desired target sites with high precision. Based on the light dependent binding of cryptochrome 2 and a cryptochrome interacting bHLH protein, we developed a split lexA transcriptional activation system for use in Drosophila that allows regulation of gene expression in vivo using blue light or two-photon excitation. We show that this system offers high spatiotemporal resolution by inducing gene expression in tissues at various developmental stages. In combination with two-photon excitation, gene expression can be manipulated at precise sites in embryos, potentially offering an important tool with which to examine developmental processes.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Regulación del Desarrollo de la Expresión Génica
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Drosophila melanogaster
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Optogenética
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Luz
Límite:
Animals
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Estados Unidos