A Peptide to Reduce Pulmonary Edema in a Rat Model of Lung Transplantation.
PLoS One
; 10(11): e0142115, 2015.
Article
en En
| MEDLINE
| ID: mdl-26536466
ABSTRACT
BACKGROUND:
Despite significant advances in organ preservation, surgical techniques and perioperative care, primary graft dysfunction is a serious medical problem in transplantation medicine in general and a specific problem in patients undergoing lung transplantation. As a result, patients develop lung edema, causing reduced tissue oxygenation capacity, reduced lung compliance and increased requirements for mechanical ventilatory support. Yet, there is no effective strategy available to protect the grafted organ from stress reactions induced by ischemia/reperfusion and by the surgical procedure itself.METHODS:
We assessed the effect of a cingulin-derived peptide, XIB13 or a random peptide in an established rat model of allogeneic lung transplantation. Donor lungs and recipients received therapeutic peptide at the time of transplantation and outcome was analyzed 100min and 28 days post grafting.RESULTS:
XIB13 improved blood oxygenation and reduced vascular leak 100min post grafting. Even after 28 days, lung edema was significantly reduced by XIB13 and lungs had reduced fibrotic or necrotic zones. Moreover, the induction of an allogeneic T cell response was delayed indicating a reduced antigen exchange between the donor and the host.CONCLUSIONS:
In summary, we provide a new tool to strengthen endothelial barrier function thereby improving outcomes in lung transplantation.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fragmentos de Péptidos
/
Edema Pulmonar
/
Resistencia Vascular
/
Trasplante de Pulmón
/
Modelos Animales de Enfermedad
/
Disfunción Primaria del Injerto
/
Proteínas de la Membrana
/
Proteínas de Microfilamentos
Tipo de estudio:
Etiology_studies
Límite:
Animals
Idioma:
En
Revista:
PLoS One
Asunto de la revista:
CIENCIA
/
MEDICINA
Año:
2015
Tipo del documento:
Article
País de afiliación:
Austria