The voltage-gated proton channel Hv1 is expressed in pancreatic islet ß-cells and regulates insulin secretion.
Biochem Biophys Res Commun
; 468(4): 746-51, 2015 Dec 25.
Article
en En
| MEDLINE
| ID: mdl-26559003
ABSTRACT
The voltage-gated proton channel Hv1 is a potent acid extruder that participates in the extrusion of the intracellular acid. Here, we showed for the first time, Hv1 is highly expressed in mouse and human pancreatic islet ß-cells, as well as ß-cell lines. Imaging studies demonstrated that Hv1 resides in insulin-containing granules in ß-cells. Knockdown of Hv1 with RNA interference significantly reduces glucose- and K(+)-induced insulin secretion in isolated islets and INS-1 (832/13) ß-cells and has an impairment on glucose- and K(+)-induced intracellular Ca(2+) homeostasis. Our data demonstrated that the expression of Hv1 in pancreatic islet ß-cells regulates insulin secretion through regulating Ca(2+) homeostasis.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Activación del Canal Iónico
/
Calcio
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Células Secretoras de Insulina
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Glucosa
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Insulina
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Canales Iónicos
Límite:
Animals
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Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2015
Tipo del documento:
Article