Effect of Dynamic Culture and Periodic Compression on Human Mesenchymal Stem Cell Proliferation and Chondrogenesis.
Ann Biomed Eng
; 44(7): 2103-13, 2016 07.
Article
en En
| MEDLINE
| ID: mdl-26577256
ABSTRACT
We have recently developed a bioreactor that can apply both shear and compressive forces to engineered tissues in dynamic culture. In our system, alginate hydrogel beads with encapsulated human mesenchymal stem cells (hMSCs) were cultured under different dynamic conditions while subjected to periodic, compressive force. A customized pressure sensor was developed to track the pressure fluctuations when shear forces and compressive forces were applied. Compared to static culture, dynamic culture can maintain a higher cell population throughout the study. With the application of only shear stress, qRT-PCR and immunohistochemistry revealed that hMSCs experienced less chondrogenic differentiation than the static group. The second study showed that chondrogenic differentiation was enhanced by additional mechanical compression. After 14 days, alcian blue staining showed more extracellular matrix formed in the compression group. The upregulation of the positive chondrogenic markers such as Sox 9, aggrecan, and type II collagen were demonstrated by qPCR. Our bioreactor provides a novel approach to apply mechanical forces to engineered cartilage. Results suggest that a combination of dynamic culture with proper mechanical stimulation may promote efficient progenitor cell expansion in vitro, thereby allowing the culture of clinically relevant articular chondrocytes for the treatment of articular cartilage defects.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Antígenos de Diferenciación
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Regulación de la Expresión Génica
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Técnicas de Cultivo de Célula
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Fuerza Compresiva
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Condrogénesis
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Proliferación Celular
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Células Madre Mesenquimatosas
Límite:
Humans
Idioma:
En
Revista:
Ann Biomed Eng
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos