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NKp46+ Innate Lymphoid Cells Dampen Vaginal CD8 T Cell Responses following Local Immunization with a Cholera Toxin-Based Vaccine.
Luci, Carmelo; Bekri, Selma; Bihl, Franck; Pini, Jonathan; Bourdely, Pierre; Nouhen, Kelly; Malgogne, Angélique; Walzer, Thierry; Braud, Véronique M; Anjuère, Fabienne.
Afiliación
  • Luci C; Université de Nice Sophia Antipolis, Institut de Pharmacologie Moléculaire et Cellulaire, Sophia Antipolis, France.
  • Bekri S; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7275, Sophia Antipolis, France.
  • Bihl F; Institut National de la Santé et de la Recherche Médicale, Institut de Pharmacologie Moléculaire et Cellulaire, France.
  • Pini J; Université de Nice Sophia Antipolis, Institut de Pharmacologie Moléculaire et Cellulaire, Sophia Antipolis, France.
  • Bourdely P; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7275, Sophia Antipolis, France.
  • Nouhen K; Université de Nice Sophia Antipolis, Institut de Pharmacologie Moléculaire et Cellulaire, Sophia Antipolis, France.
  • Malgogne A; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7275, Sophia Antipolis, France.
  • Walzer T; Université de Nice Sophia Antipolis, Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7370, Laboratoire de PhysioMédecine Moléculaire, Nice, France.
  • Braud VM; Université de Nice Sophia Antipolis, Institut de Pharmacologie Moléculaire et Cellulaire, Sophia Antipolis, France.
  • Anjuère F; Centre National de la Recherche Scientifique, Unité Mixte de Recherche 7275, Sophia Antipolis, France.
PLoS One ; 10(12): e0143224, 2015.
Article en En | MEDLINE | ID: mdl-26630176
ABSTRACT
Innate and adaptive immune cells work in concert to generate efficient protection at mucosal surface. Vaginal mucosa is an epithelial tissue that contains innate and adaptive immune effector cells. Our previous studies demonstrated that vaginal administration of Cholera toxin -based vaccines generate antigen-specific CD8 T cells through the stimulation of local dendritic cells (DC). Innate lymphoid cells (ILC) are a group of lymphocytes localized in epithelial tissues that have important immune functions against pathogens and in tissue homeostasis. Their contribution to vaccine-induced mucosal T cell responses is an important issue for the design of protective vaccines. We report here that the vaginal mucosa contains a heterogeneous population of NKp46+ ILC that includes conventional NK cells and ILC1-like cells. We show that vaginal NKp46+ ILC dampen vaccine-induced CD8 T cell responses generated after local immunization. Indeed, in vivo depletion of NKp46+ ILC with anti-NK1.1 antibody or NKG2D blockade increases the magnitude of vaginal OVA-specific CD8 T cells. Furthermore, such treatments also increase the number of DC in the vagina. NKG2D ligands being expressed by vaginal DC but not by CD8 T cells, these results support that NKp46+ ILC limit mucosal CD8 T cell responses indirectly through the NKG2D-dependent elimination of vaginal DC. Our data reveal an unappreciated role of NKp46+ ILC in the regulation of mucosal CD8 T cell responses.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Vagina / Vacunas / Antígenos Ly / Toxina del Cólera / Vacunación / Linfocitos T CD8-positivos / Receptor 1 Gatillante de la Citotoxidad Natural / Inmunidad Innata Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Francia

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Vagina / Vacunas / Antígenos Ly / Toxina del Cólera / Vacunación / Linfocitos T CD8-positivos / Receptor 1 Gatillante de la Citotoxidad Natural / Inmunidad Innata Límite: Animals Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2015 Tipo del documento: Article País de afiliación: Francia