[Effects of Paris polyphylla saponin VII plus silica nano composite on ovarian cancer drug resistance in vitro].

OBJECTIVE: To explore the drug resistance of Chonglou saponin VII plus silica nano complex for ovarian cancer. METHODS: The method of thiazolyl blue tetrazolium blue (MTT) was employed for determining the proliferation inhibition rate of Chonglou saponin VII plus silica nano composite for SKOV3/DDP drug-resistant human ovarian cancer cell. Flow cytometry and Hoechst 33342 staining were used for detecting apoptosis rate and cell cycle phase distribution. And the changes of apoptosis-related protein were examined by immunoblot assay. RESULTS: There were obvious inhibitory effects of Chonglou saponin VII on cell proliferation. The inhibition rate increased with rising drug concentration with an obvious dose-effect relationship (P < 0.05). At the same time, the cell proliferation inhibition rate significantly improved at 48 h versus 24 h (P < 0.05). Flow cytometry and Hoechst 33342 staining showed that, after a 24 h treatment of SKOV3/DDP cells with 50 and 100 µmol/L Chonglou saponin VII, early apoptotic cell ratio significantly increased compared with control group. CONCLUSION: The composite of Paris polyphylla saponin VII and silica nano has obvious proliferation inhibitory effects on SKOV3/DDP cells. And there is a significant concentration dependence and it also has some correlation with time. The above composite can increased the proportion of early apoptotic cell ratio.