Different cytokine profiles of skin-derived T cell cultures from patients with atopic dermatitis and psoriasis.
Inflamm Res
; 65(4): 265-72, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-26722669
ABSTRACT
OBJECTIVES:
To investigate differences in expression of surface markers, cytokine profiles, and presence of CD4(+)CD8(+) T cells in skin-derived T cell cultures from patients with extrinsic atopic dermatitis (AD), intrinsic AD, and psoriasis expanded in the presence of IL-2 and IL-4.MATERIAL:
Skin biopsies from patients with extrinsic AD (n = 6), intrinsic AD (n = 9) and psoriasis (n = 9).METHODS:
Skin-derived T cell cultures were analyzed for expression of six surface markers, 11 intracellular cytokines, and three T cell subtype signature transcription factors by flow cytometry, and secreted cytokines by multiplex.RESULTS:
A different IFN-γ profile emerged between the extrinsic AD and psoriatic T cell cultures; however, there was no difference in IL-17 profile. No differences with regard to cytokine expression were found between extrinsic AD and intrinsic AD cultures; however, cutaneous lymphocyte-associated antigen was expressed by a higher percentage of CD8(+) than CD4(+) T cells in the intrinsic AD cultures. Double-positive CD4(+)CD8(+) T cells were only detected in two out of 15 AD cultures.CONCLUSION:
The data suggest that IL-2 and IL-4 affects the cytokine profile during culture. Earlier findings of substantial levels of double-positive CD4(+)CD8(+) T cells in skin derived T cell cultures from AD skin was not reproduced in this study.Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Psoriasis
/
Linfocitos T
/
Citocinas
/
Dermatitis Atópica
Límite:
Adult
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Female
/
Humans
/
Male
/
Middle aged
Idioma:
En
Revista:
Inflamm Res
Asunto de la revista:
ALERGIA E IMUNOLOGIA
/
PATOLOGIA
Año:
2016
Tipo del documento:
Article
País de afiliación:
Dinamarca