A critical role of LAMP-1 in avian reovirus P10 degradation associated with inhibition of apoptosis and virus release.
Arch Virol
; 161(4): 899-911, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-26744063
ABSTRACT
Avian reovirus (ARV) causes viral arthritis, chronic respiratory diseases, retarded growth and malabsorption syndrome. The ARV p10 protein, a viroporin responsible for the induction of cell syncytium formation and apoptosis, is rapidly degraded in host cells. However, the mechanism of p10 degradation and its relevance are still unclear. We report here the identification of cellular lysosome-associated membrane protein 1 (LAMP-1) as an interaction partner of p10 by yeast two-hybrid screening, immunoprecipitation and confocal microscopy assays. We found that rapid degradation of p10 was associated with ubiquitination. Importantly, ARV p10 degradation in host cells could be completely abolished by knockdown of LAMP-1 by siRNA, indicating that LAMP-1 is required for ARV p10 degradation in host cells. In contrast, overexpression of LAMP-1 facilitated p10 degradation. Furthermore, knockdown of LAMP-1 allowed p10 accumulation, enhancing p10-induced apoptosis and viral release. Thus, LAMP-1 plays a critical role in ARV p10 degradation associated with inhibition of apoptosis and viral release.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Virales
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Apoptosis
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Orthoreovirus Aviar
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Proteína 1 de la Membrana Asociada a los Lisosomas
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Liberación del Virus
Tipo de estudio:
Risk_factors_studies
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Arch Virol
Año:
2016
Tipo del documento:
Article
País de afiliación:
China