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[Effect of antibacterial peptide hCAP18/LL-37 on ovarian cancer microenvironment and the regulatory mechanism of its expression].
Lu, Qian; Quan, Wenqiang; Wu, Junlu; Zhang, Xian; Ma, Wei; Pang, Li; Li, Dong.
Afiliación
  • Lu Q; Department of Clinical Laboratory, Tongji Hospital of Tonji University, Shanghai 200065, China.
  • Quan W; Department of Clinical Laboratory, Tongji Hospital of Tonji University, Shanghai 200065, China.
  • Wu J; Department of Clinical Laboratory, Tongji Hospital of Tonji University, Shanghai 200065, China.
  • Zhang X; Department of Clinical Laboratory, Tongji Hospital of Tonji University, Shanghai 200065, China.
  • Ma W; Department of Clinical Laboratory, Tongji Hospital of Tonji University, Shanghai 200065, China.
  • Pang L; Department of Clinical Laboratory, Tongji Hospital of Tonji University, Shanghai 200065, China.
  • Li D; Department of Clinical Laboratory, Tongji Hospital of Tonji University, Shanghai 200065, China; Email: 186ld@163.com.
Zhonghua Zhong Liu Za Zhi ; 37(10): 725-30, 2015 Oct.
Article en Zh | MEDLINE | ID: mdl-26813589
OBJECTIVE: To investigate the effect of antibacterial peptide hCAP18/LL-37 on ovarian cancer microenvironment and the regulatory mechanism of its expression. METHODS: We assessed the effect of macrophage-promoted ovarian cancer cells invasion using BioCoat Matrigel invasion chamber. The expressions of hCAP18/LL-37 and versican V1 were determined by real-time PCR and Western blot analysis. SKOV3 cells were transfected with shRNA plasmid to abrogate the expression of versican V1, and then the expression of hCAP18/LL-37 in macrophages and the invasiveness of SKOV3 cells were assayed. RESULTS: The Matrigel invasion assay showed that after co-culture with macrophages for 4 days, the number of penetrated SKOV3 cells was 112.8±17.1/per high power field, significantly higher than that in the SKOV3 cells cultured alone (8.2±1.9/per high power field) (P<0.05). Addition of hCAP/LL-37 neutralizing antibody into the co-cultured macrophage-SKOV3 cells markedly inhibited the macrophage-promoted SKOV3 cells invasion. The penetrated SKOV3 cells was 22.2±5.6/per high power field, significantly lower than the 100.6±25.2/per high power field in the control macrophage- SKOV3 co-cultured cells (P<0.05). The expressions of hCAP18/LL-37 mRNA and protein in macrophages were remarkably enhanced upon co-culture with SKOV3 cells, but not changed in SKOV3 cells cultured alone. The expression and secretion of versican V1 in the ovarian cancer cells were also significantly increased after co-cultured with macrophages. Knockdown of versican V1 in SKOV3 cells by small interfering RNA significantly reduced the expression of hCAP18/LL-37 mRNA and protein in the macrophages, as well as decreased the invasiveness of SKOV3 cells (P<0.05). CONCLUSIONS: In the cancer microenvironment, the macrophage-secreted hCAP18/LL-37 promote the invasiveness of ovarian cancer cells, and the hCAP18/LL-37 expression is regulated by versican V1 protein released by ovarian cancer cells.
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Banco de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Péptidos Catiónicos Antimicrobianos / Versicanos / Microambiente Tumoral / Proteínas de Neoplasias Límite: Female / Humans Idioma: Zh Revista: Zhonghua Zhong Liu Za Zhi Año: 2015 Tipo del documento: Article País de afiliación: China
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Banco de datos: MEDLINE Asunto principal: Neoplasias Ováricas / Péptidos Catiónicos Antimicrobianos / Versicanos / Microambiente Tumoral / Proteínas de Neoplasias Límite: Female / Humans Idioma: Zh Revista: Zhonghua Zhong Liu Za Zhi Año: 2015 Tipo del documento: Article País de afiliación: China