Single-domain intrabodies against hepatitis C virus core inhibit viral propagation and core-induced NFκB activation.
J Gen Virol
; 97(4): 887-892, 2016 Apr.
Article
en En
| MEDLINE
| ID: mdl-26861864
ABSTRACT
Hepatitis C virus (HCV) core plays a key role in viral particle formation and is involved in viral pathogenesis. Here, constructs for single-domain intrabodies consisting of variable regions derived from mouse mAbs against HCV core were established. Expressed single-domain intrabodies were shown to bind to HCV core, and inhibit the growth of cell culture-produced HCV derived from JFH-1 (genotype 2a) and a TH (genotype 1b)/JFH-1 chimera. Adenovirus vectors expressing intrabodies were also capable of reducing HCV propagation. Intrabody expression did not affect viral entry or genome replication of single-round infectious trans-complemented HCV particles. However, intrabody expression reduced intracellular and extracellular infectious titres in CD81-defective Huh7-25 cells transfected with the HCV genome, suggesting that these intrabodies impair HCV assembly. Furthermore, intrabody expression suppressed HCV core-induced NFκB promoter activity. These intrabodies may therefore serve as tools for elucidating the role of core in HCV pathogenesis.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas del Núcleo Viral
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Hepacivirus
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Hepatocitos
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Anticuerpos de Dominio Único
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Anticuerpos Monoclonales
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Anticuerpos Antivirales
Límite:
Animals
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Humans
Idioma:
En
Revista:
J Gen Virol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón