Regulation of Transcription Factor Yin Yang 1 by SET7/9-mediated Lysine Methylation.
Sci Rep
; 6: 21718, 2016 Feb 23.
Article
en En
| MEDLINE
| ID: mdl-26902152
ABSTRACT
Yin Yang 1 (YY1) is a multifunctional transcription factor shown to be critical in a variety of biological processes. Although it is regulated by multiple types of post-translational modifications (PTMs), whether YY1 is methylated, which enzyme methylates YY1, and hence the functional significance of YY1 methylation remains completely unknown. Here we reported the first methyltransferase, SET7/9 (KMT7), capable of methylating YY1 at two highly conserved lysine (K) residues, K173 and K411, located in two distinct domains, one in the central glycine-rich region and the other in the very carboxyl-terminus. Functional studies revealed that SET7/9-mediated YY1 methylation regulated YY1 DNA-binding activity both in vitro and at specific genomic loci in cultured cells. Consistently, SET7/9-mediated YY1 methylation was shown to involve in YY1-regulated gene transcription and cell proliferation. Our findings revealed a novel regulatory strategy, methylation by lysine methyltransferase, imposed on YY1 protein, and linked YY1 methylation with its biological functions.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Transcripción Genética
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Procesamiento Proteico-Postraduccional
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N-Metiltransferasa de Histona-Lisina
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Factor de Transcripción YY1
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Lisina
Límite:
Humans
Idioma:
En
Revista:
Sci Rep
Año:
2016
Tipo del documento:
Article
País de afiliación:
China