Effect of adenovirus-mediated RNA interference of IL-1ß expression on spinal cord injury in rats.
Spinal Cord
; 54(10): 778-784, 2016 Oct.
Article
en En
| MEDLINE
| ID: mdl-26902461
ABSTRACT
STUDY DESIGN:
We introduced an adenoviral vector expressing interleukin-1ß (IL-1ß) small-hairpin RNA (shRNA) into the injured spinal cords to evaluate the therapeutic potential of IL-1ß downregulation in a rat model of spinal cord injury (SCI).OBJECTIVES:
The purpose of this study was to investigate the possible protective effects of the IL-1ß downregulation on traumatic SCI in rats.SETTING:
Department of Orthopedic Surgery, The Second Affiliated Hospital, Fujian Medical University, Quanzhou, People's Republic of China.METHODS:
An adenoviral shRNA targeting IL-1ß was constructed and injected at the T12 section 7 days before SCI. The rats' motor functions were evaluated by the Basso-Beattie-Bresnahan (BBB) rating scale. Immunofluorescence, enzyme-linked immunosorbent assay, flow-cytometric analysis and western blots were also performed.RESULTS:
Animals downregulating IL-1ß had significantly better recovery of locomotor function and less neuronal loss after SCI. In addition, IL-1ß downregulation significantly decreased tumor necrosis factor-alpha (TNF-α) level and Bax expression, reduced the activity of caspase-3 and increased Bcl-2 expression after SCI.CONCLUSION:
This study demonstrated that the IL-1ß downregulation may have potential therapeutic benefits for both reducing secondary damages and improving the outcomes after traumatic SCI.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Traumatismos de la Médula Espinal
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Regulación hacia Abajo
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Interleucina-18
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Interferencia de ARN
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Spinal Cord
Asunto de la revista:
NEUROLOGIA
Año:
2016
Tipo del documento:
Article