Screening of Riboflavin-Producing Lactobacilli by a Polymerase-Chain-Reaction-Based Approach and Microbiological Assay.
J Agric Food Chem
; 64(9): 1950-6, 2016 Mar 09.
Article
en En
| MEDLINE
| ID: mdl-26902872
ABSTRACT
Riboflavin has an important role in various cellular metabolic activities through its participation in oxidation-reduction reactions. In this study, as many as 60 lactobacilli were screened for the presence or absence of riboflavin biosynthesis genes and riboflavin production. Of these, only 14 strains were able to grow in a commercial riboflavin-free medium. We observed that the presence of riboflavin biosynthesis genes is strain-specific across different species of lactobacilli. The microbiological assay was found to be appreciably reproducible, sensitive, rapid, and inexpensive and, hence, can be employed for screening the riboflavin-producing strains. The study thus represents a convenient and efficient method for selection of novel riboflavin producers. These riboflavin(+) strains thus identified and characterized could be explored as potent candidates for the development of a wide range of dairy- and cereal-based foods for the delivery of in situ riboflavin to consumers.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Riboflavina
/
Reacción en Cadena de la Polimerasa
/
Lactobacillus
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
J Agric Food Chem
Año:
2016
Tipo del documento:
Article
País de afiliación:
India