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Inducible targeting of cDCs and their subsets in vivo.
Loschko, Jakob; Rieke, Gereon J; Schreiber, Heidi A; Meredith, Matthew M; Yao, Kai-Hui; Guermonprez, Pierre; Nussenzweig, Michel C.
Afiliación
  • Loschko J; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA. Electronic address: jloschko@rockefeller.edu.
  • Rieke GJ; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA; Rheinische Friedrich-Wilhelms University Bonn, 53113 Bonn, Germany.
  • Schreiber HA; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
  • Meredith MM; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
  • Yao KH; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
  • Guermonprez P; The laboratory of phagocyte immunobiology, King's College London, UK; Centre for Molecular and Cellular Biology of Inflammation, King's College London, UK; Peter Gorer Department of Immmunobiology, King's College London, UK.
  • Nussenzweig MC; Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA; Howard Hughes Medical Institute, USA.
J Immunol Methods ; 434: 32-8, 2016 07.
Article en En | MEDLINE | ID: mdl-27073171
Conventional dendritic cells (cDCs) are essential immune cells linking the innate and adaptive immune system. cDC depletion in mice is an important method to study the function of these cells in vivo. Here we report an inducible in vivo system for cDC depletion in which excision of a loxP flanked Stop signal enables expression of the human diphtheria toxin receptor (DTR) under the control of Zbtb46 (zDC(lSlDTR)). cDCs can be specifically depleted by combining zDC(lSlDTR) mice with a Csf1r(Cre) driver line. In addition, we show that zDC(Cre) mice can be used to produce cDC specific conditional knockout mice (Irf8, Irf4, Notch2) which lack specific subsets of cDCs.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Dendríticas / Células de la Médula Ósea / Factor de Crecimiento Similar a EGF de Unión a Heparina Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Immunol Methods Año: 2016 Tipo del documento: Article
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Células Dendríticas / Células de la Médula Ósea / Factor de Crecimiento Similar a EGF de Unión a Heparina Tipo de estudio: Prognostic_studies Límite: Animals / Humans Idioma: En Revista: J Immunol Methods Año: 2016 Tipo del documento: Article