Generation of an inducible fibroblast cell line for studying direct cardiac reprogramming.
Genesis
; 54(7): 398-406, 2016 07.
Article
en En
| MEDLINE
| ID: mdl-27194122
ABSTRACT
Direct reprogramming of fibroblasts into induced cardiomyocytes (iCMs) through forced expression of cardiac-lineage specific transcription factors holds promise as an alternative strategy for cardiac regeneration. To facilitate research in iCM reprogramming, we generated a suite of new tools. We developed a transformed cell line derived from mouse embryonic fibroblasts (MEF). This fibroblast cell line (MEF-T) harbors an αMHC-eGFP reporter transgene for rapid detection of newly derived iCMs. The MEF-T cell line is highly proliferative and easily transfected and transduced, making it an ideal tool for transgene expression and genetic manipulation. Additionally, we generated a Tet-On inducible polycistronic iCM reprogramming construct for the temporal regulation of reprogramming factor expression. Furthermore, we introduced this construct into MEF-T and created an inducible reprogrammable fibroblast cell line. These tools will facilitate future research in cell fate reprogramming by enabling the temporal control of reprogramming factor expression as well as high-throughput screening using libraries of small molecules, noncoding RNAs, and siRNAs. genesis 54398-406, 2016. © 2016 Wiley Periodicals, Inc.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Regeneración
/
Diferenciación Celular
/
Miocitos Cardíacos
/
Reprogramación Celular
Límite:
Animals
Idioma:
En
Revista:
Genesis
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article