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Antiepileptic effect of fisetin in iron-induced experimental model of traumatic epilepsy in rats in the light of electrophysiological, biochemical, and behavioral observations.
Das, Jharana; Singh, Rameshwar; Sharma, Deepak.
Afiliación
  • Das J; a Laboratory of Neurobiology, School of Life Sciences, Jawaharlal Nehru University , New Delhi 110067 , India.
  • Singh R; a Laboratory of Neurobiology, School of Life Sciences, Jawaharlal Nehru University , New Delhi 110067 , India.
  • Sharma D; a Laboratory of Neurobiology, School of Life Sciences, Jawaharlal Nehru University , New Delhi 110067 , India.
Nutr Neurosci ; 20(4): 255-264, 2017 May.
Article en En | MEDLINE | ID: mdl-27198489
BACKGROUND: Traumatic epilepsy is defined by episodes of recurring seizures secondary to severe brain injury. Though drugs are found effective to control seizures, their long-term use have been observed to increase reactive oxygen species in animals. Flavonoid fisetin, a natural bioactive phytonutrient reported to exert anticonvulsive effect in experimental seizure models. But, trauma-induced seizures could not be prevented by anticonvulsants was reported in some clinical studies. OBJECTIVE: To study the effect of fisetin on epileptiform electrographic activity in iron-induced traumatic epilepsy and also the probable reason behind the effect in rats. METHODS: Fisetin pretreatment (20 mg/kg body wt., p.o.) of rats for 12 weeks were chosen followed by injecting iron (5 µl, 100 mM) stereotaxically to generate iron-induced epilepsy. Experimental design include electrophysiological study (electroencephalograph in correlation with multiple unit activity (MUA) in the cortex and CA1 subfield of the hippocampus; spectral analysis of seizure and seizure-associated behavioral study (Morris water maze for spatial learning, open-field test for anxiety) and biochemical study (lipid peroxidation, Na+,K+-ATPase activity) in both the cortex and the hippocampus. RESULTS: Fisetin pretreatment was found to prevent the development of iron-induced electrical seizure and decrease the corresponding MUA in the cortex (*P˂0.05) as well as in the hippocampus (***P˂0.001). Fisetin pretreatment decreased the lipid peroxides (*P˂0.05) and retained the Na+,K+-ATPase activity (*P˂0.05) which was found altered in the epileptic animals and also found to attenuate the seizure-associated cognitive dysfunctions. CONCLUSION: This study demonstrated the antiepileptic action of fisetin in iron-induced model of epileptic rats by inhibiting oxidative stress.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Conducta Animal / Flavonoides / Epilepsia Postraumática / Fenómenos Electrofisiológicos / Anticonvulsivantes Límite: Animals Idioma: En Revista: Nutr Neurosci Asunto de la revista: CIENCIAS DA NUTRICAO / NEUROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: India

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Conducta Animal / Flavonoides / Epilepsia Postraumática / Fenómenos Electrofisiológicos / Anticonvulsivantes Límite: Animals Idioma: En Revista: Nutr Neurosci Asunto de la revista: CIENCIAS DA NUTRICAO / NEUROLOGIA Año: 2017 Tipo del documento: Article País de afiliación: India