Study on the interaction between three benzimidazole anthelmintics and eosin Y by high performance liquid chromatography associating with resonance light scattering and its application.
Anal Bioanal Chem
; 408(19): 5247-59, 2016 Jul.
Article
en En
| MEDLINE
| ID: mdl-27209593
ABSTRACT
A novel, highly selective, and sensitive resonance light scattering (RLS) detection approach coupled with high performance liquid chromatography (HPLC) was researched and developed for the synchronous analysis of three kinds of benzimidazole anthelmintics, including mebendazole (MBZ), albendazole (ABZ), and fenbendazole (FBZ) for the first time. In the pH range of 3.5-3.7 Britton-Robinson buffer medium, three kinds of anthelmintics, which were separated by HPLC, reacted with eosin Y (EY) to form 11 ion-association complexes, resulting in significantly enhanced RLS signals and the maximum peak located at 335 nm. The enhanced RLS intensity was in proportion to the MBZ, ABZ, and FBZ concentration in the range 0.2-25, 0.2-23, and 0.15-20 µg/mL, respectively. The limit of detection was in the range of 0.064-0.16 µg/mL. In addition, human urine was determined to validate the proposed method by spiked samples and real urine samples. Satisfactory results were obtained by HPLC-RLS method. Graphical Abstract The diagram mechanism of generating resonance between emitted light and scattered light.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Refractometría
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Orina
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Bencimidazoles
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Cromatografía Líquida de Alta Presión
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Eosina Amarillenta-(YS)
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Antihelmínticos
Tipo de estudio:
Diagnostic_studies
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Evaluation_studies
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Risk_factors_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Bioanal Chem
Año:
2016
Tipo del documento:
Article