Quantitative assessment of fluorescent proteins.
Nat Methods
; 13(7): 557-62, 2016 07.
Article
en En
| MEDLINE
| ID: mdl-27240257
ABSTRACT
The advent of fluorescent proteins (FPs) for genetic labeling of molecules and cells has revolutionized fluorescence microscopy. Genetic manipulations have created a vast array of bright and stable FPs spanning blue to red spectral regions. Common to autofluorescent FPs is their tight ß-barrel structure, which provides the rigidity and chemical environment needed for effectual fluorescence. Despite the common structure, each FP has unique properties. Thus, there is no single 'best' FP for every circumstance, and each FP has advantages and disadvantages. To guide decisions about which FP is right for a given application, we have quantitatively characterized the brightness, photostability, pH stability and monomeric properties of more than 40 FPs to enable straightforward and direct comparison between them. We focus on popular and/or top-performing FPs in each spectral region.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Espectrometría de Fluorescencia
/
Proteínas Recombinantes de Fusión
/
Proteínas Luminiscentes
/
Microscopía Fluorescente
Límite:
Humans
Idioma:
En
Revista:
Nat Methods
Asunto de la revista:
TECNICAS E PROCEDIMENTOS DE LABORATORIO
Año:
2016
Tipo del documento:
Article
País de afiliación:
Estados Unidos