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Generation of reactive astrocytes from NG2 cells is regulated by sonic hedgehog.
Honsa, Pavel; Valny, Martin; Kriska, Jan; Matuskova, Hana; Harantova, Lenka; Kirdajova, Denisa; Valihrach, Lukas; Androvic, Peter; Kubista, Mikael; Anderova, Miroslava.
Afiliación
  • Honsa P; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Valny M; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Kriska J; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Matuskova H; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Harantova L; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Kirdajova D; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Valihrach L; Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Androvic P; Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Kubista M; Laboratory of Gene Expression, Institute of Biotechnology, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
  • Anderova M; Department of Cellular Neurophysiology, Institute of Experimental Medicine, Academy of Sciences of the Czech Republic, Prague, 142 20, Czech Republic.
Glia ; 64(9): 1518-31, 2016 09.
Article en En | MEDLINE | ID: mdl-27340757
ABSTRACT
NG2 cells, a fourth glial cell type in the adult mammalian central nervous system, produce oligodendrocytes in the healthy nervous tissue, and display wide differentiation potential under pathological conditions, where they could give rise to reactive astrocytes. The factors that control the differentiation of NG2 cells after focal cerebral ischemia (FCI) are largely unknown. Here, we used transgenic Cspg4-cre/Esr1/ROSA26Sortm14(CAG-tdTomato) mice, in which tamoxifen administration triggers the expression of red fluorescent protein (tomato) specifically in NG2 cells and cells derived therefrom. Differentiation potential (in vitro and in vivo) of tomato-positive NG2 cells from control or postischemic brains was determined using the immunohistochemistry, single cell RT-qPCR and patch-clamp method. The ischemic injury was induced by middle cerebral artery occlusion, a model of FCI. Using genetic fate-mapping method, we identified sonic hedgehog (Shh) as an important factor that influences differentiation of NG2 cells into astrocytes in vitro. We also manipulated Shh signaling in the adult mouse brain after FCI. Shh signaling activation significantly increased the number of astrocytes derived from NG2 cells in the glial scar around the ischemic lesion, while Shh signaling inhibition caused the opposite effect. Since Shh signaling modifications did not change the proliferation rate of NG2 cells, we can conclude that Shh has a direct influence on the differentiation of NG2 cells and therefore, on the formation and composition of a glial scar, which consequently affects the degree of the brain damage. GLIA 2016;641518-1531.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Encéfalo / Diferenciación Celular / Oligodendroglía / Neuroglía / Astrocitos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Glia Asunto de la revista: NEUROLOGIA Año: 2016 Tipo del documento: Article País de afiliación: República Checa

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Encéfalo / Diferenciación Celular / Oligodendroglía / Neuroglía / Astrocitos Tipo de estudio: Prognostic_studies Límite: Animals Idioma: En Revista: Glia Asunto de la revista: NEUROLOGIA Año: 2016 Tipo del documento: Article País de afiliación: República Checa