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Ultra-High Resolution 3D Imaging of Whole Cells.
Huang, Fang; Sirinakis, George; Allgeyer, Edward S; Schroeder, Lena K; Duim, Whitney C; Kromann, Emil B; Phan, Thomy; Rivera-Molina, Felix E; Myers, Jordan R; Irnov, Irnov; Lessard, Mark; Zhang, Yongdeng; Handel, Mary Ann; Jacobs-Wagner, Christine; Lusk, C Patrick; Rothman, James E; Toomre, Derek; Booth, Martin J; Bewersdorf, Joerg.
Afiliación
  • Huang F; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Weldon School of Biomedical Engineering, Purdue University, West Lafayette, IN 47907, USA.
  • Sirinakis G; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.
  • Allgeyer ES; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; The Gurdon Institute, University of Cambridge, Cambridge CB2 1QN, UK.
  • Schroeder LK; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Duim WC; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Department of Chemistry, Harvey Mudd College, Claremont, CA 91711, USA.
  • Kromann EB; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Department of Biomedical Engineering, Yale University, CT 06520, USA.
  • Phan T; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Rivera-Molina FE; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Myers JR; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Irnov I; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA.
  • Lessard M; The Jackson Laboratory, Bar Harbor, ME 04609, USA.
  • Zhang Y; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Handel MA; The Jackson Laboratory, Bar Harbor, ME 04609, USA.
  • Jacobs-Wagner C; Microbial Sciences Institute, Yale University, West Haven, CT 06516, USA; Department of Molecular, Cellular and Developmental Biology, Yale University, New Haven, CT 06520, USA; Howard Hughes Medical Institute, Yale University, New Haven, CT 06520, USA; Department of Microbial Pathogenesis, Yale Sch
  • Lusk CP; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA.
  • Rothman JE; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Nanobiology Institute, Yale University, West Haven, CT 06516, USA.
  • Toomre D; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Nanobiology Institute, Yale University, West Haven, CT 06516, USA.
  • Booth MJ; Department of Engineering Science, University of Oxford, Oxford OX1 3PJ, UK; Centre for Neural Circuits and Behaviour, University of Oxford, Oxford OX1 3SR, UK.
  • Bewersdorf J; Department of Cell Biology, School of Medicine, Yale University, New Haven, CT 06520, USA; Department of Biomedical Engineering, Yale University, CT 06520, USA; Nanobiology Institute, Yale University, West Haven, CT 06516, USA. Electronic address: joerg.bewersdorf@yale.edu.
Cell ; 166(4): 1028-1040, 2016 Aug 11.
Article en En | MEDLINE | ID: mdl-27397506
Fluorescence nanoscopy, or super-resolution microscopy, has become an important tool in cell biological research. However, because of its usually inferior resolution in the depth direction (50-80 nm) and rapidly deteriorating resolution in thick samples, its practical biological application has been effectively limited to two dimensions and thin samples. Here, we present the development of whole-cell 4Pi single-molecule switching nanoscopy (W-4PiSMSN), an optical nanoscope that allows imaging of three-dimensional (3D) structures at 10- to 20-nm resolution throughout entire mammalian cells. We demonstrate the wide applicability of W-4PiSMSN across diverse research fields by imaging complex molecular architectures ranging from bacteriophages to nuclear pores, cilia, and synaptonemal complexes in large 3D cellular volumes.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Técnicas Citológicas / Imagen Individual de Molécula / Microscopía Fluorescente Límite: Animals Idioma: En Revista: Cell Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Técnicas Citológicas / Imagen Individual de Molécula / Microscopía Fluorescente Límite: Animals Idioma: En Revista: Cell Año: 2016 Tipo del documento: Article País de afiliación: Estados Unidos