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A data set from flash X-ray imaging of carboxysomes.
Hantke, Max F; Hasse, Dirk; Ekeberg, Tomas; John, Katja; Svenda, Martin; Loh, Duane; Martin, Andrew V; Timneanu, Nicusor; Larsson, Daniel S D; van der Schot, Gijs; Carlsson, Gunilla H; Ingelman, Margareta; Andreasson, Jakob; Westphal, Daniel; Iwan, Bianca; Uetrecht, Charlotte; Bielecki, Johan; Liang, Mengning; Stellato, Francesco; DePonte, Daniel P; Bari, Sadia; Hartmann, Robert; Kimmel, Nils; Kirian, Richard A; Seibert, M Marvin; Mühlig, Kerstin; Schorb, Sebastian; Ferguson, Ken; Bostedt, Christoph; Carron, Sebastian; Bozek, John D; Rolles, Daniel; Rudenko, Artem; Foucar, Lutz; Epp, Sascha W; Chapman, Henry N; Barty, Anton; Andersson, Inger; Hajdu, Janos; Maia, Filipe R N C.
Afiliación
  • Hantke MF; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Hasse D; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Ekeberg T; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • John K; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Svenda M; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Loh D; Centre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117543, Singapore.
  • Martin AV; ARC Centre of Excellence for Advanced Molecular Imaging, School of Physics, The University of Melbourne, Victoria 3010, Australia.
  • Timneanu N; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Larsson DS; Department of Physics and Astronomy, Uppsala University, Lägerhyddsvägen 1, Box 516, Uppsala SE- 751 20, Sweden.
  • van der Schot G; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Carlsson GH; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Ingelman M; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Andreasson J; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Westphal D; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Iwan B; ELI beamlines, Institute of Physics, Academy of Sciences of the Czech Republic, Na Slovance 2, Prague 18221, Czech Republic.
  • Uetrecht C; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Bielecki J; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Liang M; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Stellato F; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • DePonte DP; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
  • Bari S; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
  • Hartmann R; I.N.F.N. and Physics Department, University of Rome 'Tor Vergata', Via della Ricerca Scientifica 1, Rome 00133, Italy.
  • Kimmel N; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Kirian RA; European XFEL GmbH, Albert-Einstein-Ring 19, Hamburg 22761, Germany.
  • Seibert MM; Deutsches Elektronen-Synchrotron DESY, Notkestr. 85, Hamburg 22607, Germany.
  • Mühlig K; PNSensor GmbH, Römerstrasse 28, München 80803, Germany.
  • Schorb S; Max Planck Institute for Extraterrestrial Physics, Giessenbachstrasse, Garching 85741, Germany.
  • Ferguson K; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
  • Bostedt C; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Carron S; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Bozek JD; Department of Cell and Molecular Biology, Laboratory of Molecular Biophysics, Uppsala University, Husargatan 3 (Box 596), Uppsala SE-751 24, Sweden.
  • Rolles D; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Rudenko A; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Foucar L; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Epp SW; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Chapman HN; LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
  • Barty A; Center for Free-Electron Laser Science, DESY, Notkestrasse 85, Hamburg 22607, Germany.
  • Andersson I; Department of Physics, J.R. Macdonald Laboratory, Kansas State University, Cardwell Hall, Manhattan, Kansas 66506, USA.
  • Hajdu J; Max Planck Institute for Medical Research, Jahnstraße 29, Heidelberg 69120, Germany.
  • Maia FR; Max Planck Advanced Study Group at the Center for Free-Electron Laser Science (CFEL), Notkestraße 85, Hamburg 22607, Germany.
Sci Data ; 3: 160061, 2016 Aug 01.
Article en En | MEDLINE | ID: mdl-27479842
ABSTRACT
Ultra-intense femtosecond X-ray pulses from X-ray lasers permit structural studies on single particles and biomolecules without crystals. We present a large data set on inherently heterogeneous, polyhedral carboxysome particles. Carboxysomes are cell organelles that vary in size and facilitate up to 40% of Earth's carbon fixation by cyanobacteria and certain proteobacteria. Variation in size hinders crystallization. Carboxysomes appear icosahedral in the electron microscope. A protein shell encapsulates a large number of Rubisco molecules in paracrystalline arrays inside the organelle. We used carboxysomes with a mean diameter of 115±26 nm from Halothiobacillus neapolitanus. A new aerosol sample-injector allowed us to record 70,000 low-noise diffraction patterns in 12 min. Every diffraction pattern is a unique structure measurement and high-throughput imaging allows sampling the space of structural variability. The different structures can be separated and phased directly from the diffraction data and open a way for accurate, high-throughput studies on structures and structural heterogeneity in biology and elsewhere.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Orgánulos / Halothiobacillus / Ciclo del Carbono Idioma: En Revista: Sci Data Año: 2016 Tipo del documento: Article País de afiliación: Suecia
Texto completo
Imprimir
XML
PubMed Links
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Orgánulos / Halothiobacillus / Ciclo del Carbono Idioma: En Revista: Sci Data Año: 2016 Tipo del documento: Article País de afiliación: Suecia