Preparation of Colloidal Gold Particles and Conjugation to Protein A/G/L, IgG, F(ab')2, and Streptavidin.
Methods Mol Biol
; 1474: 61-71, 2016.
Article
en En
| MEDLINE
| ID: mdl-27515074
ABSTRACT
Colloidal gold probes, including protein A/G/L, IgG, F(ab')2, and streptavidin labeled with gold particles, are useful tools to localize antigens in cells and tissues by immunoelectron microscopy (IEM). This chapter describes different methods for the preparation of colloidal gold and conjugation of colloidal gold to protein A/G/L, IgG, and streptavidin.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteína Estafilocócica A
/
Proteínas Bacterianas
/
Inmunoglobulina G
/
Fragmentos Fab de Inmunoglobulinas
/
Oro Coloide
Límite:
Humans
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2016
Tipo del documento:
Article
País de afiliación:
Japón