Your browser doesn't support javascript.
loading
Prognostic Significance of Mixed-Lineage Leukemia (MLL) Gene Detected by Real-Time Fluorescence Quantitative PCR Assay in Acute Myeloid Leukemia.
Huang, Sai; Yang, Hua; Li, Yan; Feng, Cong; Gao, Li; Chen, Guo-Feng; Gao, Hong-Hao; Huang, Zhi; Li, Yong-Hui; Yu, Li.
Afiliación
  • Huang S; Department of Hematology, Chinese PLA General Hospital, Beijing, China (mainland).
  • Yang H; Department of Hematology, Chinese PLA General Hospital, Beijing, China (mainland).
  • Li Y; Department of Hematology, Chinese PLA General Hospital, Beijing, China (mainland).
  • Feng C; Department of Emergency Medicine, Chinese PLA General Hospital, Beijing, China (mainland).
  • Gao L; Department of Hematology, China-Japan Friendship Hospital, Hepingli, Beijing, China (mainland).
  • Chen GF; Department of Hematology, Chinese PLA General Hospital, Beijing, China (mainland).
  • Gao HH; Department of Hematology, Chinese PLA General Hospital, Beijing, China (mainland).
  • Huang Z; Department of Electrical and Computer Engineering, Indiana University-Purdue University Indianapolis, Indianapolis, IN, USA.
  • Li YH; Department of Hematology, Chinese PLA General Hospital, Beijing, China (mainland).
  • Yu L; Department of Hematology, Chinese PLA General Hospital, Beijing, China (mainland).
Med Sci Monit ; 22: 3009-17, 2016 Aug 26.
Article en En | MEDLINE | ID: mdl-27561414
BACKGROUND The overall prognosis of acute myeloid leukemia (AML) patients with mixed-lineage leukemia (MLL) gene-positivity is unfavorable. In this study, we evaluated the expression levels of the MLL gene in AML patients. MATERIAL AND METHODS We enrolled 68 MLL gene-positive patients out of 433 newly diagnosed AML patients, and 216 bone marrow samples were collected. Real-time fluorescence quantitative PCR (RQ-PCR) was used to precisely detect the expression levels of the MLL gene. RESULTS We divided 41 patients into 2 groups according to the variation of MRD (minimal residual disease) level of the MLL gene. Group 1 (n=22) had a rapid reduction of MRD level to ≤10^-4 in all samples collected in the first 3 chemotherapy cycles, while group 2 (n=19) had MRD levels constantly >10^-4 in all samples collected in the first 3 chemotherapy cycles. Group 1 had a significantly better overall survival (p=0.001) and event-free survival (p=0.001) compared to group 2. Moreover, the patients with >10^-4 MRD level before the start of HSCT (hematopoietic stem cell transplantation) had worse prognosis and higher risk of relapse compared to patients with ≤10^-4 before the start of HSCT. CONCLUSIONS We found that a rapid reduction of MRD level to ≤10^-4 appears to be a prerequisite for better overall survival and event-free survival during the treatment of AML. The MRD levels detected by RQ-PCR were basically in line with the clinical outcome and may be of great importance in guiding early allogeneic HSCT (allo-HSCT) treatment.
Asunto(s)

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / N-Metiltransferasa de Histona-Lisina / Proteína de la Leucemia Mieloide-Linfoide Tipo de estudio: Prognostic_studies Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Med Sci Monit Asunto de la revista: MEDICINA Año: 2016 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Leucemia Mieloide Aguda / N-Metiltransferasa de Histona-Lisina / Proteína de la Leucemia Mieloide-Linfoide Tipo de estudio: Prognostic_studies Límite: Adolescent / Adult / Aged / Female / Humans / Male / Middle aged Idioma: En Revista: Med Sci Monit Asunto de la revista: MEDICINA Año: 2016 Tipo del documento: Article