Assembling and auditing a comprehensive DNA barcode reference library for European marine fishes.
J Fish Biol
; 89(6): 2741-2754, 2016 Dec.
Article
en En
| MEDLINE
| ID: mdl-27739061
ABSTRACT
A large-scale comprehensive reference library of DNA barcodes for European marine fishes was assembled, allowing the evaluation of taxonomic uncertainties and species genetic diversity that were otherwise hidden in geographically restricted studies. A total of 4118 DNA barcodes were assigned to 358 species generating 366 Barcode Index Numbers (BIN). Initial examination revealed as much as 141 BIN discordances (more than one species in each BIN). After implementing an auditing and five-grade (A-E) annotation protocol, the number of discordant species BINs was reduced to 44 (13% grade E), while concordant species BINs amounted to 271 (78% grades A and B) and 14 other had insufficient data (grade D). Fifteen species displayed comparatively high intraspecific divergences ranging from 2·6 to 18·5% (grade C), which is biologically paramount information to be considered in fish species monitoring and stock assessment. On balance, this compilation contributed to the detection of 59 European fish species probably in need of taxonomic clarification or re-evaluation. The generalized implementation of an auditing and annotation protocol for reference libraries of DNA barcodes is recommended.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Biblioteca de Genes
/
Código de Barras del ADN Taxonómico
/
Peces
Límite:
Animals
País/Región como asunto:
Europa
Idioma:
En
Revista:
J Fish Biol
Año:
2016
Tipo del documento:
Article
País de afiliación:
Portugal