Exploration of ZEA cytotoxicity to mouse endometrial stromal cells and RNA-seq analysis.

ABSTRACT

Because zearalenone (ZEA) causes harmful influence to animals and widely exists in the world, the researches on ZEA have never stopped. However, the mechanisms of ZEA on proliferation, cycle, and apoptosis in endometrial stromal cells (ESCs) remain poorly defined. Therefore, the purpose of our study was to explore the effects of ZEA to ESCs and demonstrate them by transcriptomic analysis. The results showed that after ZEA treatment, ESCs appeared numerous adverse reactions, and the phenomena of cell viability decrease, DNA replication block and apoptosis were detected by flow cytometry, Annexin V-FITC/PI double-staining method, TUNEL assay, and so on. Then, RNA-seq approach was adopted to prove the validity of above experiments, as expected, the results from different expression genes, gene ontology terms, and KEGG pathway were all consistent with those. Overall, the results suggested that ZEA could cause a series of reactions by cytotoxicity to mouse ESCs, meanwhile there must be some substances and mechanisms protect cells against cytotoxicity damage.