Effects of storage time on the motility, mortality and calcium levels of Atlantic salmon Salmo salar spermatozoa.
J Fish Biol
; 90(4): 1506-1516, 2017 Apr.
Article
en En
| MEDLINE
| ID: mdl-28067414
ABSTRACT
This study estimates spermatozoa mortality, morphology, motility and intracellular calcium levels in Atlantic salmon Salmo salar milt after prolonged storage. Milt samples were preserved at 4° C for 25 days and then evaluated for mortality. Motility remained high for the first 3 days and the mortality was low during the first 5 days of storage. A decrease of >50% in calcium content was observed after 5 days of storage. When spermatozoa were activated, calcium levels increased >200% in relative fluorescence units (RFU); this rate of increase was lost when the samples were stored for extended periods of time and was only partially manifested in a zero calcium solution. The results suggest that in vitro storage of S. salar spermatozoa at 4° C for a period of 3 days preserves motility and limits mortality to levels similar to those of fresh spermatozoa. This method also maintains intracellular calcium storage critical for spermatozoa performance.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Preservación de Semen
/
Motilidad Espermática
/
Calcio
/
Salmo salar
Límite:
Animals
Idioma:
En
Revista:
J Fish Biol
Año:
2017
Tipo del documento:
Article
País de afiliación:
Chile