[Effect of triptolide on human oral cancer cell proliferation and PTEN gene mRNA expression in oral cancer].
Zhonghua Kou Qiang Yi Xue Za Zhi
; 52(1): 44-47, 2017 Jan 09.
Article
en Zh
| MEDLINE
| ID: mdl-28072994
ABSTRACT
Objective:
To investigate the effect of triptolide on human oral cancer cell (HB) proliferation and phosphates and tensin homologue deleted on chromosome ten gene (PTEN) mRNA expression in oral cancer.Methods:
The cancer cells were cultured in the medium containing triptolide of different concentrations for 24, 48 and 72 h. Methyl thiazolyl tetrazolium (MTT) method was used to test the rate of growth inhibition of cancer cells, flow cytometer to detect the change of cell cycle and reveres transcription-PCR (RT-PCR) to examine the expression of PTEN mRNA. The expression of PTEN protein was examined by Western blotting.Results:
The rate of growth inhibition was (26.92 ± 0.14)%, (38.67 ± 0.11)%, (72.62 ± 0.89)% and (90.42 ± 0.28)%, respectively. The corresponding expression of PTEN mRNA was (3.59±0.21)%, (5.27±0.40)%, (7.18±0.44)% and (9.16±0.50)%, respectively and the corresponding A value of PTEN protein was 0.135±0.007, 0.410±0.020, 0.447±0.017 and 0.884±0.066, respectively. The proportion of G1 phase cells increased from (58.78±0.98)% to (84.13±0.47)%, but the proportion of S phase cells decreased from (25.40±0.43)% to (9.41±0.73)%.Conclusions:
The triptolide not only had inhibitory effect on the HB proliferation, but also affected the cell cycle.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Fenantrenos
/
ARN Mensajero
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Neoplasias de la Boca
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Antineoplásicos Alquilantes
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Proliferación Celular
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Diterpenos
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Fosfohidrolasa PTEN
Límite:
Humans
Idioma:
Zh
Revista:
Zhonghua Kou Qiang Yi Xue Za Zhi
Asunto de la revista:
ODONTOLOGIA
Año:
2017
Tipo del documento:
Article
País de afiliación:
China