Boosted large-scale production and purification of a thermostable archaeal phosphotriesterase-like lactonase for organophosphate decontamination.

Restaino, Odile Francesca; Borzacchiello, Maria Giovanna; Scognamiglio, Ilaria; Porzio, Elena; Manco, Giuseppe; Fedele, Luigi; Donatiello, Cinzia; De Rosa, Mario; Schiraldi, Chiara

Restaino, Odile Francesca; Borzacchiello, Maria Giovanna; Scognamiglio, Ilaria; Porzio, Elena; Manco, Giuseppe; Fedele, Luigi; Donatiello, Cinzia; De Rosa, Mario; Schiraldi, Chiara.

Afiliación

Restaino OF; Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Naples, Italy. odilefrancesca.restaino@unina2.it.
Borzacchiello MG; Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Naples, Italy.
Scognamiglio I; Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Naples, Italy.
Porzio E; Institute of Protein Biochemistry, National Research Council of Italy, Naples, Italy.
Manco G; Institute of Protein Biochemistry, National Research Council of Italy, Naples, Italy.
Fedele L; Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Naples, Italy.
Donatiello C; Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Naples, Italy.
De Rosa M; Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Naples, Italy.
Schiraldi C; Department of Experimental Medicine, Section of Biotechnology and Molecular Biology, Second University of Naples, Naples, Italy. chiara.schiraldi@unina2.it.

J Ind Microbiol Biotechnol ; 44(3): 363-375, 2017 03.

Article en En | MEDLINE | ID: mdl-28074318

Asunto(s)

Hidrolasas de Éster Carboxílico/biosíntesis; Genes Arqueales; Organofosfatos/química; Hidrolasas de Triéster Fosfórico/biosíntesis; Sulfolobus solfataricus/genética; Técnicas de Cultivo Celular por Lotes; Medios de Cultivo/química; Descontaminación; Escherichia coli/genética; Escherichia coli/metabolismo; Fermentación; Microbiología Industrial; Microorganismos Modificados Genéticamente; Ingeniería de Proteínas; Sulfolobus solfataricus/metabolismo

Palabras clave

High cell density cultivation; Organophosphates; Sulfolobus solfataricus; Thermostable phosphotriesterase-like lactonase; Ultrafiltration

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Organofosfatos / Hidrolasas de Éster Carboxílico / Genes Arqueales / Hidrolasas de Triéster Fosfórico / Sulfolobus solfataricus Idioma: En Revista: J Ind Microbiol Biotechnol Asunto de la revista: BIOTECNOLOGIA / MICROBIOLOGIA Año: 2017 Tipo del documento: Article País de afiliación: Italia

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