High-fidelity <i>Glucagon-CreER</i> mouse line generated by CRISPR-Cas9 assisted gene targeting.

Ackermann, Amanda M; Zhang, Jia; Heller, Aryel; Briker, Anna; Kaestner, Klaus H

High-fidelity Glucagon-CreER mouse line generated by CRISPR-Cas9 assisted gene targeting.

Ackermann, Amanda M; Zhang, Jia; Heller, Aryel; Briker, Anna; Kaestner, Klaus H.

Afiliación

Ackermann AM; Division of Endocrinology and Diabetes, The Children's Hospital of Philadelphia, 3400 Civic Center Boulevard, Philadelphia, PA, 19104, USA; Institute of Diabetes, Obesity, and Metabolism, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA, 1910
Zhang J; Institute of Diabetes, Obesity, and Metabolism, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA, 19104, USA; Department of Genetics, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA,
Heller A; Institute of Diabetes, Obesity, and Metabolism, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA, 19104, USA; Department of Genetics, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA,
Briker A; Department of Genetics, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA, 19104, USA. Electronic address: abriker@villanova.edu.
Kaestner KH; Institute of Diabetes, Obesity, and Metabolism, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA, 19104, USA; Department of Genetics, Perelman School of Medicine, The University of Pennsylvania, 3400 Civic Center Boulevard, Philadelphia, PA,

Mol Metab ; 6(3): 236-244, 2017 03.

Article en En | MEDLINE | ID: mdl-28271030

Asunto(s)

Ingeniería Genética/métodos; Glucagón/genética; Ratones Transgénicos/genética; Regiones no Traducidas 3'/genética; Animales; Sistemas CRISPR-Cas/efectos de los fármacos; Diabetes Mellitus Tipo 2/genética; Diabetes Mellitus Tipo 2/metabolismo; Técnicas de Sustitución del Gen; Marcación de Gen; Técnicas Genéticas; Glucagón/metabolismo; Células Secretoras de Glucagón/metabolismo; Células Secretoras de Glucagón/fisiología; Islotes Pancreáticos/efectos de los fármacos; Ratones; Tamoxifeno/farmacología; Transgenes/efectos de los fármacos

Palabras clave

CRISPR; CRISPR, clustered regularly interspaced short palindromic repeat; Cre, Cre recombinase; CreERT2, tamoxifen-inducible Cre recombinase-estrogen receptor fusion protein; DAPI, 4',6-diamidino-2-phenylindole; ESC, embryonic stem cell; Enteroendocrine L-cell; FACS, fluorescence-activated cell sorting; GCG, glucagon; GLP1; GLP1, glucagon-like peptide 1; Glucagon; IRES, internal ribosomal entry site; Islet; LSL, loxP-stop-loxP; UTR, untranslated region; YFP, yellow fluorescent protein; gRNA, guide RNA; α-cell

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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Glucagón / Ratones Transgénicos / Ingeniería Genética Límite: Animals Idioma: En Revista: Mol Metab Año: 2017 Tipo del documento: Article

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