Highly Sensitive Glucose Sensor Based on Organic Electrochemical Transistor with Modified Gate Electrode.
Methods Mol Biol
; 1572: 205-216, 2017.
Article
en En
| MEDLINE
| ID: mdl-28299690
ABSTRACT
An organic electrochemical transistor (OECT) with a glucose oxidase (GOx) and poly(n-vinyl-2-pyrrolidone)-capped platinum nanoparticles (Pt NPs) gate electrode was successfully integrated with a microfluidic channel to act as a highly sensitive chip-based glucose sensor. The sensing mechanism relies on the enzymatic reaction between glucose and GOx followed by electrochemical oxidation of hydrogen peroxide (H2O2) produced in the enzymatic reaction. This process largely increases the electrolyte potential that applies on PEDOTPSS channel and causes more cations penetrate into PEDOTPSS film to reduce it to semi-conducting state resulting in lower electric current between the source and the drain. The extremely high sensitivity and low detection limit (0.1 µM) of the sensor was achievable due to highly efficient Pt NPs catalysis in oxidation of H2O2. Pt NPs were deposited by a bias-free two-step dip coating method followed by a UV-Ozone post-treatment to enhance catalytic ability. A polydimethylsiloxane (PDMS) microfluidic channel was directly attached to the OECT active layer, providing a short detection time (~1 min) and extremely low analyte consumption (30 µL). Our sensor has great potential for real-time, noninvasive, and portable glucose sensing applications due to its compact size and high sensitivity.
Palabras clave
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Técnicas Biosensibles
/
Electrodos
/
Técnicas Electroquímicas
/
Glucosa
Tipo de estudio:
Diagnostic_studies
Idioma:
En
Revista:
Methods Mol Biol
Asunto de la revista:
BIOLOGIA MOLECULAR
Año:
2017
Tipo del documento:
Article
País de afiliación:
China