Single molecule mtDNA fiber FISH for analyzing numtogenesis.
Anal Biochem
; 552: 45-49, 2018 07 01.
Article
en En
| MEDLINE
| ID: mdl-28322800
Somatic human cells contain thousands of copies of mitochondrial DNA (mtDNA). In eukaryotes, natural transfer of mtDNA into the nucleus generates nuclear mitochondrial DNA (NUMT) copies. We name this phenomenon as "numtogenesis". Numtogenesis is a well-established evolutionary process reported in various sequenced eukaryotic genomes. We have established a molecular tool to rapidly detect and analyze NUMT insertions in whole genomes. To date, NUMT analyses depend on deep genome sequencing combined with comprehensive computational analyses of the whole genome. This is time consuming, cumbersome and cost prohibitive. Further, most laboratories cannot accomplish such analyses due to limited skills. We report the development of single-molecule mtFIBER FISH (fluorescence in situ hybridization) to study numtogenesis. The development of mtFIBER FISH should aid in establishing a role for numtogenesis in cancers and other human diseases. This novel technique should help distinguish and monitor cancer stages and progression, aid in elucidation of basic mechanisms underlying tumorigenesis and facilitate analyses of processes related to early detection of cancer, screening and/or cancer risk assessment.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
ADN Mitocondrial
/
Núcleo Celular
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Hibridación Fluorescente in Situ
Tipo de estudio:
Risk_factors_studies
/
Screening_studies
Límite:
Humans
Idioma:
En
Revista:
Anal Biochem
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos