Using Synthetic Biology to Engineer Living Cells That Interface with Programmable Materials.
J Vis Exp
; (121)2017 03 09.
Article
en En
| MEDLINE
| ID: mdl-28362372
ABSTRACT
We have developed an abiotic-biotic interface that allows engineered cells to control the material properties of a functionalized surface. This system is made by creating two modules a synthetically engineered strain of E. coli cells and a functionalized material interface. Within this paper, we detail a protocol for genetically engineering selected behaviors within a strain of E. coli using molecular cloning strategies. Once developed, this strain produces elevated levels of biotin when exposed to a chemical inducer. Additionally, we detail protocols for creating two different functionalized surfaces, each of which is able to respond to cell-synthesized biotin. Taken together, we present a methodology for creating a linked, abiotic-biotic system that allows engineered cells to control material composition and assembly on nonliving substrates.
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1
Banco de datos:
MEDLINE
Asunto principal:
Escherichia coli
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Biología Sintética
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Ingeniería Celular
Idioma:
En
Revista:
J Vis Exp
Año:
2017
Tipo del documento:
Article