Your browser doesn't support javascript.
loading
Whole genome sequencing of Shigella sonnei through PulseNet Latin America and Caribbean: advancing global surveillance of foodborne illnesses.
Baker, K S; Campos, J; Pichel, M; Della Gaspera, A; Duarte-Martínez, F; Campos-Chacón, E; Bolaños-Acuña, H M; Guzmán-Verri, C; Mather, A E; Diaz Velasco, S; Zamudio Rojas, M L; Forbester, J L; Connor, T R; Keddy, K H; Smith, A M; López de Delgado, E A; Angiolillo, G; Cuaical, N; Fernández, J; Aguayo, C; Morales Aguilar, M; Valenzuela, C; Morales Medrano, A J; Sirok, A; Weiler Gustafson, N; Diaz Guevara, P L; Montaño, L A; Perez, E; Thomson, N R.
Afiliación
  • Baker KS; University of Liverpool, Department of Functional and Comparative Genomics, Liverpool, England, United Kingdom; Wellcome Trust Sanger Institute, Pathogen Variation Programme, Hinxton, England, United Kingdom. Electronic address: kbaker@liverpool.ac.uk.
  • Campos J; Instituto Nacional de Enfermedades Infecciosas, ANLIS, Buenos Aires, Argentina.
  • Pichel M; Instituto Nacional de Enfermedades Infecciosas, ANLIS, Buenos Aires, Argentina.
  • Della Gaspera A; Instituto Nacional de Enfermedades Infecciosas, ANLIS, Buenos Aires, Argentina.
  • Duarte-Martínez F; Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud (Inciensa), Costa Rica.
  • Campos-Chacón E; Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud (Inciensa), Costa Rica.
  • Bolaños-Acuña HM; Instituto Costarricense de Investigación y Enseñanza en Nutrición y Salud (Inciensa), Costa Rica.
  • Guzmán-Verri C; Programa de Investigación en Enfermedades Tropicales, Escuela de Medicina Veterinaria, Universidad Nacional, Heredia, Costa Rica; Centro de Investigación en Enfermedades Tropicales, Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica.
  • Mather AE; Wellcome Trust Sanger Institute, Pathogen Variation Programme, Hinxton, England, United Kingdom; University of Cambridge, Department of Veterinary Medicine, Cambridge, England, United Kingdom.
  • Diaz Velasco S; National Institute of Heath, Lima, Peru.
  • Zamudio Rojas ML; National Institute of Heath, Lima, Peru.
  • Forbester JL; Wellcome Trust Sanger Institute, Pathogen Variation Programme, Hinxton, England, United Kingdom.
  • Connor TR; Organisms and Environment Division, Cardiff University School of Biosciences, Sir Martin Evans Building, Cardiff, Wales, United Kingdom.
  • Keddy KH; Centre for Enteric Diseases, National Institute for Communicable Diseases and Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • Smith AM; Centre for Enteric Diseases, National Institute for Communicable Diseases and Faculty of Health Sciences, University of the Witwatersrand, Johannesburg, South Africa.
  • López de Delgado EA; Department of Bacteriology, National Institute of Hygiene 'Rafael Rangel', Ciudad University, Los Chaguaramos, Venezuela.
  • Angiolillo G; Department of Bacteriology, National Institute of Hygiene 'Rafael Rangel', Ciudad University, Los Chaguaramos, Venezuela.
  • Cuaical N; Department of Bacteriology, National Institute of Hygiene 'Rafael Rangel', Ciudad University, Los Chaguaramos, Venezuela.
  • Fernández J; Molecular Genetics Laboratory, Institute of Public Health of Chile, Santiago, Chile.
  • Aguayo C; Molecular Genetics Laboratory, Institute of Public Health of Chile, Santiago, Chile.
  • Morales Aguilar M; Department of Foodborne Diseases, National Health Laboratory of Guatemala, Laboratorio Nacional de Salud, Barcenas, Guatemala.
  • Valenzuela C; Department of Foodborne Diseases, National Health Laboratory of Guatemala, Laboratorio Nacional de Salud, Barcenas, Guatemala.
  • Morales Medrano AJ; Department of Foodborne Diseases, National Health Laboratory of Guatemala, Laboratorio Nacional de Salud, Barcenas, Guatemala.
  • Sirok A; Bacteriology Laboratory, Departamento de Laboratorios de Salud Pública (DLSP), Ministerio de Salud Pública (MSP), Montevideo, Uruguay.
  • Weiler Gustafson N; Department of Bacteriology, Laboratorio Central de Salud Pública, Asuncion, Paraguay.
  • Diaz Guevara PL; Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.
  • Montaño LA; Grupo de Microbiología, Instituto Nacional de Salud, Bogotá, Colombia.
  • Perez E; Pan American Health Organization/World Health Organization, Department of Health Emergencies, Washington, DC, United States.
  • Thomson NR; Wellcome Trust Sanger Institute, Pathogen Variation Programme, Hinxton, England, United Kingdom; London School of Hygiene and Tropical Medicine, London, England, United Kingdom. Electronic address: nrt@sanger.ac.uk.
Clin Microbiol Infect ; 23(11): 845-853, 2017 Nov.
Article en En | MEDLINE | ID: mdl-28389276
OBJECTIVES: Shigella sonnei is a globally important diarrhoeal pathogen tracked through the surveillance network PulseNet Latin America and Caribbean (PNLA&C), which participates in PulseNet International. PNLA&C laboratories use common molecular techniques to track pathogens causing foodborne illness. We aimed to demonstrate the possibility and advantages of transitioning to whole genome sequencing (WGS) for surveillance within existing networks across a continent where S. sonnei is endemic. METHODS: We applied WGS to representative archive isolates of S. sonnei (n = 323) from laboratories in nine PNLA&C countries to generate a regional phylogenomic reference for S. sonnei and put this in the global context. We used this reference to contextualise 16 S. sonnei from three Argentinian outbreaks, using locally generated sequence data. Assembled genome sequences were used to predict antimicrobial resistance (AMR) phenotypes and identify AMR determinants. RESULTS: S. sonnei isolates clustered in five Latin American sublineages in the global phylogeny, with many (46%, 149 of 323) belonging to previously undescribed sublineages. Predicted multidrug resistance was common (77%, 249 of 323), and clinically relevant differences in AMR were found among sublineages. The regional overview showed that Argentinian outbreak isolates belonged to distinct sublineages and had different epidemiologic origins. CONCLUSIONS: Latin America contains novel genetic diversity of S. sonnei that is relevant on a global scale and commonly exhibits multidrug resistance. Retrospective passive surveillance with WGS has utility for informing treatment, identifying regionally epidemic sublineages and providing a framework for interpretation of prospective, locally sequenced outbreaks.
Asunto(s)
Palabras clave

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Shigella sonnei / Disentería Bacilar / Enfermedades Transmitidas por los Alimentos Tipo de estudio: Observational_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Clin Microbiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2017 Tipo del documento: Article

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Shigella sonnei / Disentería Bacilar / Enfermedades Transmitidas por los Alimentos Tipo de estudio: Observational_studies / Risk_factors_studies / Screening_studies Límite: Humans Idioma: En Revista: Clin Microbiol Infect Asunto de la revista: DOENCAS TRANSMISSIVEIS / MICROBIOLOGIA Año: 2017 Tipo del documento: Article