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Genome-Wide Comparison of Next-Generation Sequencing and qPCR Platforms for microRNA Profiling in Serum.
Blondal, Thorarinn; Brunetto, Maurizia Rossana; Cavallone, Daniela; Mikkelsen, Martin; Thorsen, Michael; Mang, Yuan; Pinheiro, Hazel; Bonino, Ferruccio; Mouritzen, Peter.
Afiliación
  • Blondal T; Exiqon A/S, Vedbaek, Denmark.
  • Brunetto MR; Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Pisa, Italy.
  • Cavallone D; Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Pisa, Italy.
  • Mikkelsen M; Exiqon A/S, Vedbaek, Denmark.
  • Thorsen M; Exiqon A/S, Vedbaek, Denmark.
  • Mang Y; Exiqon A/S, Vedbaek, Denmark.
  • Pinheiro H; Exiqon A/S, Vedbaek, Denmark.
  • Bonino F; Hepatology Unit and Laboratory of Molecular Genetics and Pathology of Hepatitis Viruses, Reference Center of the Tuscany Region for Chronic Liver Disease and Cancer, University Hospital of Pisa, Pisa, Italy.
  • Mouritzen P; Exiqon A/S, Vedbaek, Denmark. pm@exiqon.com.
Methods Mol Biol ; 1580: 21-44, 2017.
Article en En | MEDLINE | ID: mdl-28439824
ABSTRACT
This study compares next-generation sequencing (NGS) technologies that have been optimized specifically for biofluid samples, with more established qPCR-based methods for profiling microRNAs in biofluids. The same patient serum samples were analyzed by NGS and qPCR, and differences in the serum microRNA profile between HBV and HCV infected patients were investigated. While there was overall good agreement between NGS and qPCR, there were some differences between the platforms, highlighting the importance of validation.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Perfilación de la Expresión Génica / MicroARNs / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Perfilación de la Expresión Génica / MicroARNs / Secuenciación de Nucleótidos de Alto Rendimiento Límite: Humans Idioma: En Revista: Methods Mol Biol Asunto de la revista: BIOLOGIA MOLECULAR Año: 2017 Tipo del documento: Article País de afiliación: Dinamarca