Full shut-off of Escherichia coli RNA-polymerase by T7 phage requires a small phage-encoded DNA-binding protein.
Nucleic Acids Res
; 45(13): 7697-7707, 2017 Jul 27.
Article
en En
| MEDLINE
| ID: mdl-28486695
ABSTRACT
Infection of Escherichia coli by the T7 phage leads to rapid and selective inhibition of the bacterial RNA polymerase (RNAP) by the 7 kDa T7 protein Gp2. We describe the identification and functional and structural characterisation of a novel 7 kDa T7 protein, Gp5.7, which adopts a winged helix-turn-helix-like structure and specifically represses transcription initiation from host RNAP-dependent promoters on the phage genome via a mechanism that involves interaction with DNA and the bacterial RNAP. Whereas Gp2 is indispensable for T7 growth in E. coli, we show that Gp5.7 is required for optimal infection outcome. Our findings provide novel insights into how phages fine-tune the activity of the host transcription machinery to ensure both successful and efficient phage progeny development.
Texto completo:
1
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Virales
/
ARN Polimerasas Dirigidas por ADN
/
Bacteriófago T7
/
Proteínas de Escherichia coli
/
Proteínas de Unión al ADN
/
Escherichia coli
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Nucleic Acids Res
Año:
2017
Tipo del documento:
Article
País de afiliación:
Reino Unido