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Two Silene vulgaris copper transporters residing in different cellular compartments confer copper hypertolerance by distinct mechanisms when expressed in Arabidopsis thaliana.
Li, Yanbang; Iqbal, Mazhar; Zhang, Qianqian; Spelt, Cornelis; Bliek, Mattijs; Hakvoort, Henk W J; Quattrocchio, Francesca M; Koes, Ronald; Schat, Henk.
Afiliación
  • Li Y; Swammerdam Institute for Life Sciences, Department of Plant Development and (Epi)Genetics, University of Amsterdam, 1098XH, Amsterdam, the Netherlands.
  • Iqbal M; Department of Molecular and Cell Biology, Faculty of Earth and Life Sciences, VU-University, De Boelelaan 1108, 1081 HZ, Amsterdam, the Netherlands.
  • Zhang Q; Swammerdam Institute for Life Sciences, Department of Plant Development and (Epi)Genetics, University of Amsterdam, 1098XH, Amsterdam, the Netherlands.
  • Spelt C; Swammerdam Institute for Life Sciences, Department of Plant Cell Biology, University of Amsterdam, 1098XH, Amsterdam, the Netherlands.
  • Bliek M; Swammerdam Institute for Life Sciences, Department of Plant Development and (Epi)Genetics, University of Amsterdam, 1098XH, Amsterdam, the Netherlands.
  • Hakvoort HWJ; Department of Molecular and Cell Biology, Faculty of Earth and Life Sciences, VU-University, De Boelelaan 1108, 1081 HZ, Amsterdam, the Netherlands.
  • Quattrocchio FM; Swammerdam Institute for Life Sciences, Department of Plant Development and (Epi)Genetics, University of Amsterdam, 1098XH, Amsterdam, the Netherlands.
  • Koes R; Department of Molecular and Cell Biology, Faculty of Earth and Life Sciences, VU-University, De Boelelaan 1108, 1081 HZ, Amsterdam, the Netherlands.
  • Schat H; Department of Molecular and Cell Biology, Faculty of Earth and Life Sciences, VU-University, De Boelelaan 1108, 1081 HZ, Amsterdam, the Netherlands.
New Phytol ; 215(3): 1102-1114, 2017 Aug.
Article en En | MEDLINE | ID: mdl-28620999
Silene vulgaris is a metallophyte of calamine, cupriferous and serpentine soils all over Europe. Its metallicolous populations are hypertolerant to zinc (Zn), cadmium (Cd), copper (Cu) or nickel (Ni), compared with conspecific nonmetallicolous populations. These hypertolerances are metal-specific, but the underlying mechanisms are poorly understood. We investigated the role of HMA5 copper transporters in Cu-hypertolerance of a S. vulgaris copper mine population. Cu-hypertolerance in Silene is correlated and genetically linked with enhanced expression of two HMA5 paralogs, SvHMA5I and SvHMA5II, each of which increases Cu tolerance when expressed in Arabidopsis thaliana. Most Spermatophytes, except Brassicaceae, possess homologs of SvHMA5I and SvHMA5II, which originate from an ancient duplication predating the appearance of spermatophytes. SvHMA5II and the A. thaliana homolog AtHMA5 localize in the endoplasmic reticulum and upon Cu exposure move to the plasma membrane, from where they are internalized and degraded in the vacuole. This resembles trafficking of mammalian homologs and is apparently an extremely ancient mechanism. SvHMA5I, instead, neofunctionalized and always resides on the tonoplast, likely sequestering Cu in the vacuole. Adaption of Silene to a Cu-polluted soil is at least in part due to upregulation of two distinct HMA5 transporters, which contribute to Cu hypertolerance by distinct mechanisms.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Proteínas de Plantas / Adaptación Fisiológica / Arabidopsis / Cobre / Silene Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Proteínas de Transporte de Membrana / Proteínas de Plantas / Adaptación Fisiológica / Arabidopsis / Cobre / Silene Idioma: En Revista: New Phytol Asunto de la revista: BOTANICA Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos