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Pulmonary vascular changes in extremely preterm sheep after intra-amniotic exposure to Ureaplasma parvum and lipopolysaccharide.
Willems, Monique G M; Kemp, Matthew W; Fast, Laura A; Wagemaker, Nick M M; Janssen, Leon E W; Newnham, John P; Payne, Matt S; Spiller, Owen B; Kallapur, Suhas G; Jobe, Alan H; Delhaas, Tammo; Kramer, Boris W; Wolfs, Tim G A M.
Afiliación
  • Willems MGM; Department of Pediatrics, Maastricht University Medical Center, Maastricht, The Netherlands.
  • Kemp MW; Department of BioMedical Engineering, Maastricht University Medical Center, Maastricht, The Netherlands.
  • Fast LA; GROW School for Oncology and Developmental Biology, Maastricht University Medical Center, Maastricht, The Netherlands.
  • Wagemaker NMM; School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia.
  • Janssen LEW; Department of Pediatrics, Maastricht University Medical Center, Maastricht, The Netherlands.
  • Newnham JP; Department of Pediatrics, Maastricht University Medical Center, Maastricht, The Netherlands.
  • Payne MS; Department of Pediatrics, Maastricht University Medical Center, Maastricht, The Netherlands.
  • Spiller OB; School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia.
  • Kallapur SG; School of Women's and Infants' Health, The University of Western Australia, Perth, Western Australia, Australia.
  • Jobe AH; School of Medicine, Cardiff University, University Hospital of Wales, Cardiff, United Kingdom.
  • Delhaas T; Division of Neonatology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, Unites States of America.
  • Kramer BW; Division of Neonatology, Cincinnati Children's Hospital Medical Center, Cincinnati, Ohio, Unites States of America.
  • Wolfs TGAM; Department of BioMedical Engineering, Maastricht University Medical Center, Maastricht, The Netherlands.
PLoS One ; 12(6): e0180114, 2017.
Article en En | MEDLINE | ID: mdl-28666032
BACKGROUND: Chorioamnionitis can induce pulmonary inflammation and promote bronchopulmonary dysplasia development, distinguished by alveolar simplification and impaired vascular growth. Chorioamnionitis is more common during the extremely preterm canalicular lung stage (crucial for vascular development); and increases the risk for subsequent sepsis. We hypothesized that single/combined exposure to chronic and/or acute inflammation induces pulmonary inflammatory responses and vascular changes. METHODS: Ovine fetuses were intra-amniotically exposed to chronic Ureaplasma parvum (UP) at 24 days (d) before extreme preterm delivery at 94d (term 147d) and/or to lipopolysaccharide (LPS) 7 or 2d before delivery. Pulmonary inflammation, vascular remodeling and angiogenic factors were assessed. RESULTS: LPS exposure increased CD3-positive and myeloperoxidase-positive cells. Combined UP-LPS exposure increased pulmonary inflammation compared with 2d LPS or UP groups. The UP+2d LPS group had an increased adventitial fibrosis score when compared with UP-treated animals. A reduced wall-to-lumen ratio was found in the 7d LPS animals when compared to the 2d LPS-treated animals. Exposure to UP+2d LPS reduced VEGF and VEGFR-2 levels compared with 2d LPS-treated animals. Angiopoietin-1 (Ang1) and tunica interna endothelial cell kinase 2 (Tie-2) levels were decreased after UP+7d LPS as well as after 7d LPS, but not with UP alone. CONCLUSION: Chronic UP and subsequent LPS exposure increased pulmonary inflammation and decreased expression of angiogenic growth factors and receptors when compared to single hit-exposed animals.
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Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ureaplasma / Vasos Sanguíneos / Ovinos / Lipopolisacáridos / Exposición Materna / Pulmón Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos

Texto completo: 1 Banco de datos: MEDLINE Asunto principal: Ureaplasma / Vasos Sanguíneos / Ovinos / Lipopolisacáridos / Exposición Materna / Pulmón Tipo de estudio: Prognostic_studies Límite: Animals / Pregnancy Idioma: En Revista: PLoS One Asunto de la revista: CIENCIA / MEDICINA Año: 2017 Tipo del documento: Article País de afiliación: Países Bajos